TRPC4AP Protein (AA 2-797) (His tag)

Produktnummer ABIN3137329

Produktdetails

Target: TRPC4AP (Transient Receptor Potential Cation Channel, Subfamily C, Member 4 Associated Protein (TRPC4AP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-797

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses TRPC4AP Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: AAAPAAAGAG ASRGRRLAAT AAAWGGWGGR PRPGNILLQL RQGQLTGRGL VRAVQFTETF LTERDKLSKW SGIPQLLLKL YATSHLHSDF VECQSILKEI SPLLSMEAMA FVTEDRKFTQ EATYPNTYIF DLFGGVDLLV EILMRPTISI RGQKLKISDE MSKDCLSILY NTCVCTEGVT KRLAEKNDFV IFLFTLMTSK KTFLQTATLI EDILGVKKEM IRLDEVPNLS SLVSNFDQQQ LANFCRILAV TISEMDTGND DKHTLLAKNA QQKKSLSLGP SAAEINQAAL LSIPGFVERL CKLATRKVSE STGTASFLQE LEEWYTWLDN ALVLDALMRV ANEESEHNQA PTVFPSLGTS EEGGLPHTSA RAQLPQSMKI MHEIMYKLEV LYVLCVLLMG RQRNQVHRMI AEFKLIPGLN NLFDKLIWRK HSASALVLHG HNQNCDCSPD ITLKIQFLRL LQSFSDHHEN KYLLLNNQEL NELSAISLKA NIPEVEAVLN TDRSLVCDGK RGLLTRLLQV MKKEPAESSF RFWQARAVES FLRGTTSYAD QMFLLKRGLL EHILYCIVDS ECKSRDVLQS YFDLLGELMK FNVDAFKRFN KYINTDAKFQ VFLKQINSSL VDSNMLVRCV TLSLDRFENQ VDMKVAEVLS ECRLLAYISQ VPTQMSFLFR LINIIHVQTL TQENVSCLNT SLVILMLARR KERLPLYLRL LQRMEHSKKY PGFLLNNFHN LLRFWQQHYL HKDKDSTCLE NSSCISFSYW KETVSILLNP DRQSPSALVS YIEEPYMDID RDFTEE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Trpc4ap Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TRPC4AP (Transient Receptor Potential Cation Channel, Subfamily C, Member 4 Associated Protein (TRPC4AP))

Andere Bezeichnung: Trpc4ap (TRPC4AP Produkte)

Synonyme: TRPC4AP Protein, MGC130977 Protein, 4833429F06Rik Protein, D2Ertd113e Protein, Trrp4ap Protein, mFLJ00177 Protein, truss Protein, C20orf188 Protein, TRRP4AP Protein, TRUSS Protein, transient receptor potential cation channel subfamily C member 4 associated protein Protein, transient receptor potential cation channel, subfamily C, member 4 associated protein L homeolog Protein, transient receptor potential cation channel, subfamily C, member 4 associated protein Protein, TRPC4AP Protein, trpc4ap.L Protein, trpc4ap Protein, Trpc4ap Protein

Hintergrund: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC (By similarity). Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1. {ECO:0000250, ECO:0000269|PubMed:16876162, ECO:0000269|PubMed:20458742}.

Molekulargewicht: 91.5 kDa Including tag.

UniProt: Q9JLV2