COPB1 Protein (AA 2-953) (His tag)

Produktnummer ABIN3137274

Produktdetails

Target: COPB1 (Coatomer Protein Complex, Subunit beta 1 (COPB1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-953

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses COPB1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: TAAENVCYTL INVPMDSEPP SEISLKNDLE KGDVKSKTEA LKKVIIMILN GEKLPGLLMT IIRFVLPLQD HTIKKLLLVF WEIVPKTTPD GRLLHEMILV CDAYRKDLQH PNEFIRGSTL RFLCKLKEAE LLEPLMPAIR ACLEHRHSYV RRNAVLAIYT IYRNFEHLIP DAPELIHDFL VNEKDASCKR NAFMMLIHAD QDRALDYLST CIDQVQTFGD ILQLVIVELI YKVCHANPSE RARFIRCIYN LLQSSSPAVK YEAAGTLVTL SSAPTAIKAA AQCYIDLIIK ESDNNVKLIV LDRLVELKEH PAHERVLQDL VMDILRVLST PDLEVRKKTL QLALDLVSSR NVEELVIVLK KEVIKTNNVS EHEDTDKYRQ LLVRTLHSCS VRFPDMAANV IPVLMEFLSD SNEAAAADVL EFVREAIQRF DNLRMLIVEK MLEVFHAIKS VKIYRGALWI LGEYCSTKED IQSVMTEVRR SLGEIPIVES EIKKEAGELK PEEEITVGPV QKLVTEMGTY ATQSALSSSR PTKKEEDRPP LRGFLLDGDF FVAASLATTL TKIALRYVAL VQEKKKQNSF VAEAMLLMAT ILHLGKSSLP KKPITDDDVD RISLCLKVLS ECSPLMNDIF NKECRQSLSQ MLSAKLEEEK LSQKKESEKR NVTVQPDDPI SFMQLTAKNE MNCKEDQFQL SLLAAMGNTQ RKEAADPLAS KLNKVTQLTG FSDPVYAEAY VHVNQYDIVL DVLVVNQTSD TLQNCTLELA TLGDLKLVEK PSPLTLAPHD FANIKANVKV ASTENGIIFG NIVYDVSGAA SDRNCVVLSD IHIDIMDYIQ PATCTDAEFR QMWAEFEWEN KVTVNTNMTD LNDYLQHILK STNMKCLTPE KALSGYCGFM AANLYARSIF GEDALANVSI EKPVHQGPDA AVTGHIRIRA KSQGMALSLG DKINLSQKKT SL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Copb1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: COPB1 (Coatomer Protein Complex, Subunit beta 1 (COPB1))

Andere Bezeichnung: Copb1 (COPB1 Produkte)

Synonyme: id:ibd5034 Protein, wu:fc66c05 Protein, zgc:92482 Protein, COPB1 Protein, DKFZp459K154 Protein, COPB Protein, 2610019B04Rik Protein, Rack2 Protein, Beta-COP Protein, coatomer protein complex, subunit beta 1 Protein, coatomer protein complex subunit beta 1 L homeolog Protein, coatomer protein complex subunit beta 1 Protein, copb1 Protein, copb1.L Protein, COPB1 Protein, Copb1 Protein

Hintergrund: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1 (By similarity). Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins in cortical neurons. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis. {ECO:0000250, ECO:0000269|PubMed:17698811, ECO:0000269|PubMed:19067489}.

Molekulargewicht: 107.9 kDa Including tag.

UniProt: Q9JIF7