SCYL2 Protein (AA 1-930) (Strep Tag)

Produktnummer ABIN3136432

Produktdetails

Target: SCYL2 (SCY1-Like 2 (SCYL2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-930

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SCYL2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MESMLNKLKS TVTKVTADVT SAVMGNPVTR EFDVGRHIAS GGNGLAWKIF NGTKKSTKQE VAVFVFDKKL IDKYQKFEKD QIIDSLKRGV QQLTRLRHPR LLTVQHPLEE SRDCLAFCTE PVFASLANVL GNWENLPSSI SPDIKDYKLY DVETKYGLLQ VSEGLSFLHS SVKMVHGNVT PENVILNKSG AWKIMGFDFC VSSSNPSEQE PKFPCKEWDP NLPSLCLPNP EYLAPEYILS VSCETASDMY SLGAVMYAVF NQGRPIFEVN KQDIYKSFSR QLDQLSRLGS SSLTSIPEEV REHVKLLLNV TPTVRPDADQ MTKIPFFDDV GAVTLQYFDT LFQRDNLQKS QFFKGLPKVL PKLPKRVIVQ RILPCLTSEF VNPDMVPFVL PNVLLIAEEC TKEEYIKLIL PELGPVFKQQ EPIQILLIFL QKMDLLLTKT PPDEIKNSVL PMVYRALEAP SIQIQELCLN IIPTFANLID YPSMKNALIP RIKNACLQTS SLAVRVNSLV CLGKILEYLD KWFVLDDILP FLQQIPSKEP AVLMGILGIY KCTFTHKKLG ITKEQLAGKV LPHLIPLSIE NNLNLNQFSS FIAVIKEMLS RLESEHRTKL EQLHVMQEQQ RSLDIGNQMS TSEETKVAHS GSQQIDKVFN NIGADLLSGS ESENREDGMQ GKQKRGSLTL EEKQKLAKEQ EQAQKLKSQQ PLKPQVHTPI APIKQTKDLT DTLMENMSSL TSLSVSTPKI SASSTFTPVP STGLGMMFST PIDNTKRNLT NGLNANMGFQ TSGFSMPVNP NQNFFSGTGT AGVTTMSLGA PPTMSNFSPL TIPPASVKQP QQRPTDMSAL NNLFGPQKPK VSMNQLSQQK PNQWLNQFAP PQGSPVMGSA AMGTQGNVMG QAAFGMQGNP FFNPQNFAQP PPTTMTSSSS ASNDLKDLFG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SCYL2 (SCY1-Like 2 (SCYL2))

Andere Bezeichnung: Scyl2 (SCYL2 Produkte)

Synonyme: RGD1305665 Protein, CVAK104 Protein, AU015688 Protein, AW536445 Protein, BC030932 Protein, D10Ertd802e Protein, SCY1 like pseudokinase 2 Protein, SCY1-like protein 2 Protein, SCY1-like 2 (S. cerevisiae) Protein, Scyl2 Protein, SCYL2 Protein, LOC452160 Protein

Hintergrund: SCY1-like protein 2 (Coated vesicle-associated kinase of 104 kDa),FUNCTION: Component of the AP2-containing clathrin coat that may regulate clathrin-dependent trafficking at plasma membrane, TGN and endosomal system. A possible serine/threonine-protein kinase toward the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine has not been confirmed (By similarity). By regulating the expression of excitatory receptors at synapses, plays an essential role in neuronal function and signaling and in brain development (PubMed:26203146). {ECO:0000250|UniProtKB:Q6P3W7, ECO:0000269|PubMed:26203146}.

Molekulargewicht: 103.3 kDa

UniProt: Q8CFE4