rho Guanine Nucleotide Exchange Factor (GEF) 10 (ARHGEF10) (AA 1-1345) protein (His tag)

Details zu Produkt Nr. ABIN3136335, Anbieter: Anmelden zum Anzeigen
Proteinname
  • ARHGEF10
  • wu:fi38h08
  • GEF10
  • 6430549H08Rik
  • mKIAA0294
  • Rho guanine nucleotide exchange factor 10
  • Rho guanine nucleotide exchange factor (GEF) 10
  • ARHGEF10
  • arhgef10
  • Arhgef10
Proteineigenschaft
AA 1-1345
1
1
1
1
1
Spezies
Maus
3
Quelle
Insektenzellen
2
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses ARHGEF10 Protein ist gelabelt mit His tag.
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Sequenz MEQGEASPPV PAEHEAKCDT SNNEEEGELF DFDSGDEVPE ADRQVPSADD RTRGAEAGGA DENTCPAGNG TGAEPAPEAE PAKLVVPTKV NPYSVIDITP LQEDQPSSPD ANTEEEGVGL RVPSGYSVPV PCGYAVPSNL PLLLPAYSSP VIIRAESVEE EEAAETVGDG QCNSLSSEDL PHSSEQGSQE GSALARWAAD PANTAWMENP EEAIYDDVPR ENSDSEPDEM IYDDVENGEE GGNSSPEYGW SSSEFESYEE PSDSEGKNGI PRSFLRSSHK KQLSHDLTRF KAHCEEKMRG LVASTVGAME IQQAKQRQER KMQKLMKAAK EGTKDGLEKT KAAVKRGGSF IRTRSLVSQD HRCYFEEEQN LFIDVDCKHP EAVLTPMPEG LSQQQVVRRY ILGSIVESEK NYVDALRRIL EQYEKPLSEM EPRLLSDRKL RMVFYRVKEI LQCHSMFQIA LASRVSEWDV VETIGDVFVA SFSKSMVLDA YSEYVNNFST AVAVLKKTCA TKPAFLEFLK LSQDSSPDRV TLHSLMMRPI QRFPQFILLL QDMLKNTAKG HPDRLPLQMA LTELETLAEK LNERKRDADQ RCEIKQIAKA INERYLNKLL SSGNRYLVRS DDVIETVYND RGEIVKTKQR RIFMLNDVLM CATASSRNSH ESHAVMSQRY LLKWSVPLGH VDVIQYNGGS GAGEHCRHHA AHSPESLAVV ANAKPHKVYM GPGQLYQDLQ NLLHDLNVVG QISQLIGNLR GSYQNLNQSV AHDWTSGLQR LILRKEDAIR AADRCRIQLQ LPGKQDKSGR PTFFTAVFNT LTPAIKESWV SSLQMAKLAL EEENHMGWFC VDDDGNLAKK ETHPLLVGHM PVMVAKQPEF KIECAAYNPE PYLSNESQPA SPSTARGFLW IGSCSNQMGQ VAIVSFQGSN PKVIECFNVE SRILCMVYIP AEESKPQETT ETKDPEATAS RAPHVPTICL GTEEGSISIY KSSQGCKKVR LQHFYAPDKS TVMSLACSPQ GLYAGLVNGS VASYTKAPDG SWNSEPQQVI KLGVLPVRSL LLVEGALWAA SGGQVFMASV ETHTIENQLE AHQDEGMVIS HMAVAGVGIW IAFTSGSTLR LFHTETLKHL QDVNIDAPVH SMLPGHQRLS VTSLLVCHGL LMVGTSLGVV VALPVPRLQG IPKVTGRGMV SYHAHNGPVK FIVSATAFQN KDRARDSPRS GSELQDEDPK DLLCSEEGPS CPGQPDTYTS VWLGDSLGLP TQKNDLSSSS GSLNLSHGSS SLEHRSVDSS LCDLLRDPSA SPRSRPQGSR RARASSALVV CGGQGHRRVH RKARQPSQED LVSSVMVWQI PLLGM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Arhgef10 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Reinigung Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin free.
Güteklasse Crystallography grade
Hintergrund May play a role in developmental myelination of peripheral nerves. {ECO:0000269|PubMed:14508709}.
Molekulargewicht 148.9 kDa Including tag.
UniProt Q8C033
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for rho Guanine Nucleotide Exchange Factor (GEF) 10 (ARHGEF10) (AA 1-1345) protein (His tag) (ABIN3136335) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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