RPS6KC1 Protein (AA 1-1056) (Strep Tag)

Produktnummer ABIN3136218

Produktdetails

Target: RPS6KC1 (Ribosomal Protein S6 Kinase, 52kDa, Polypeptide 1 (RPS6KC1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1056

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses RPS6KC1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MTSPWGHSAD LARFYTVTEP QRHPRGYTVY KVTARVVSRR NPEDVQEIIV WRRYSDFKKL HRELWQIHRN AFRHSELFPP FAKGTVFGRF DKTVIEERRQ CAEDLLQFSA NIPALYNSRQ LQDFFKGGVI SDGSELIGPA EAYPDSPANA FPECGTEGFS SDSDLLSLTV DADSLAEVDD GMASRQGSPS RTFGLSLSTD SSAVGAVASD SEPSRVEDRE SRSLFPSSLK PRLGRRDYLE KAGELIKLAL KKEEEDDYEA ASDFYRKGVD LLLEGVQGES SPTRREAVKR RTAEYLMRAE SICSLRAAPQ LHTGPQPPGS LSSRPPWSLR SPAEELKAFR VLGVIDKVLL VMDTRTEQTF ILKGLRKSSE CSRNRKTIIP RCVPNMVCLH TYIISEESVF LVLQRAEGGK LWSYISKFLN RSSQESLDIK EGRPSMPPRV CLQQPSASPQ GGSSFESRGS DTGSMLKALP LKTSLTPSSQ DDSNQEDDGQ PSSPKWLDSG SSSEDECTAG YLTLCNEYGQ EKMDLVSLSE ESVMQPEGDK ADTQAVSSPA SLATGSVSPS THLRVFSGGE DLEAVSSPPT SESLSRSKNS PMEFFRIDSK DSTSELLGLD FGEKLHSLKP EPLKALFTLE DGDSPSQSLD PGESKRESEA QDSVSRGSDD SVPVISFKEA AAEAISGAEE GRPDLLVNLP GELQPTKEAS AMDPKFSQAS AGRLDSKLLE APDVLCLRLS SEQCHGLGPE GPEELSDPTE FCPGGVIPEH DAQADPGVLF EAAVDHRSSP DQFLFSSLRS ESDRLGQVEV VVTAQALQES LFHISSPCSG ANKEHSAYAD TATSEEVLLF TEPTKEEANS LFQRGSEAQE RGVGAGEADK EIHQIFEDLD KRLAASSRFF IPEGCIQRWA AEMVVALDAL HREGIVCRDL NPNNILLNDG GHIQLTYFSR WSEVEDSCDS DAVARMYCAP EVGAVTEETE ACDWWSLGAV LFELLTGKTL VECHPAGINT HTTLNMPGCV SEEARSLIQQ LLQFNPMERL GAGVAGVEDI KSHPFFTPVD WAELTR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RPS6KC1 (Ribosomal Protein S6 Kinase, 52kDa, Polypeptide 1 (RPS6KC1))

Andere Bezeichnung: Rps6kc1 (RPS6KC1 Produkte)

Synonyme: GDNFRB Protein, NRTNR-ALPHA Protein, NTNRA Protein, RETL2 Protein, TRNR2 Protein, RPK118 Protein, humS6PKh1 Protein, AA682037 Protein, B130003F20Rik Protein, C80612 Protein, Rpk118 Protein, MGC81290 Protein, RPS6KC1 Protein, GDNF family receptor alpha 2 Protein, ribosomal protein S6 kinase C1 Protein, ribosomal protein S6 kinase polypeptide 1 Protein, ribosomal protein S6 kinase, 52kDa, polypeptide 1 L homeolog Protein, ribosomal protein S6 kinase delta-1 Protein, ribosomal protein S6 kinase, 52kDa, polypeptide 1 Protein, GFRA2 Protein, RPS6KC1 Protein, Rps6kc1 Protein, rps6kc1.L Protein, LOC100465753 Protein, rps6kc1 Protein

Hintergrund: Ribosomal protein S6 kinase delta-1 (S6K-delta-1) (EC 2.7.11.1) (52 kDa ribosomal protein S6 kinase),FUNCTION: May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell. Plays a role in the recruitment of PRDX3 to early endosomes. {ECO:0000250|UniProtKB:Q96S38}.

Molekulargewicht: 115.7 kDa

UniProt: Q8BLK9