SNX19 Protein (AA 1-997) (Strep Tag)

Produktnummer ABIN3135746

Produktdetails

Target: SNX19 (Sorting Nexin 19 (SNX19))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-997

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SNX19 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MKAQTVSPTQ GTISESSYVH SNLWSSRKLM IVGVLVGWLL VIHLLVNMWL LILLCASLVA LGGWLGSTAI LGASGQLHLE RFITITTCPP CPEAERQLEQ EINRTIQMII RDFVLSWYRS VSHEPAFEAE MEAAMKGLVQ ELRRRMSIVD SHALTQRVLT LCGCHLQSYI QAKEATAKEQ SCPVQPSQLW DAYCQVTAPH PAMSCPTTEV TYARGIVNLI LKELVPKPHL ETRTGRHVVV EVITCNVILP LISKLSDPDW IHLILVSIFS KYRHDAAQGT KPPCSSSVLE QPSVPTSLPL IVEVESLPVG KASSPATAPV HLTSSEPAPS PEIEEGHEAV EGDLPGMLEE KKVGNSSSHF LQPDIRGPLF LCEDSELESP LSELSKETIL LMTPGNFLSD RIQDALCALD DSGALEPKDG EGSECMEGAE AEEAPGTDTE TGMLVSVLNC PEIQIDTADK EVEQGDDTSL TALLEEPEKP CPLRPSCLDK DLASGVCSLE PAMPPVPLSS SPPGPLSSAT FSFESLSSPD GPVVIQNLRI TGTITAREHS GTGFHPYTLY TVKYETVLNG ENSSGLQQLA YHTVNRRYRE FLNLQTRLEE KPDLRKFIKN VKGPKKLFPD LPFGNMDSDR VEARKSLLES FLKQLCAIPE IGNSEEVQEF LALNTDARIA FVKKPFMVSR IDKMVVSAIV DTLKTAFPRS EPQSPTEELS EAENESKPQT EGKKASKSRL RFSSSKIAPA LSIAEAQDKI LYCLQEGNSE SEVLSMSGME SFIEKQTKLL RIQPAEVPDK DPQQVPKEYV DSGLLDKAVV AQELNKSGPG TETELADTAF DLILLLLMEQ WKWLCTESMQ KFLHIIFGTL VQRWLEVQVA NLTCPQRWAQ YLHLLRESIW PGGVLPKFPR PGRTQAQKAA TEKQALQSLM DLLPDFLVEI LGVNKCRLSW SLVLESFQQP LINRHLIYCL GDIILELLDL SASVEECAPA TSASDSPGSL KKMAVST
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SNX19 (Sorting Nexin 19 (SNX19))

Andere Bezeichnung: Snx19 (SNX19 Produkte)

Synonyme: AI195321 Protein, AI848208 Protein, mKIAA0254 Protein, 3526401K03Rik Protein, CHET8 Protein, sorting nexin 19 Protein, sorting nexin 19 S homeolog Protein, Snx19 Protein, snx19.S Protein, SNX19 Protein, snx19 Protein

Hintergrund: Sorting nexin-19,FUNCTION: Plays a role in intracellular vesicle trafficking and exocytosis (PubMed:24843546). May play a role in maintaining insulin-containing dense core vesicles in pancreatic beta-cells and in preventing their degradation. May play a role in insulin secretion (PubMed:24843546). Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) (PubMed:25148684). {ECO:0000269|PubMed:24843546, ECO:0000269|PubMed:25148684}.

Molekulargewicht: 109.8 kDa

UniProt: Q6P4T1