Hairless Protein (HR) (AA 1-1182) (His tag)

Produktnummer ABIN3135442

Produktdetails

Target: Hairless (HR)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1182

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Hairless Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: MESMPSFLKD TPAWEKTAPV NGIVGQEPGT SPQDGLRHGA LCLGEPAPFW RGVLSTPDSW LPPGFLQGPK DTLSLVEGEG PRNGERKGSW LGGKEGLRWK EAMLAHPLAF CGPACPPRYG PLIPEHSGGH PKSDPVAFRP LHCPFLLETK ILERAPFWVP TCLPPYLMSS LPPERPYDWP LAPNPWVYSG SQPKVPSAFG LGSKGFYHKD PNILRPAKEP LAESGMLGLA PGGHLQQACE SEGPSLHQRD GETGAGRQQN LCPVFLGYPD TVPRAPWPSC PPGLVHSLGN IWAGPGSNSL GYQLGPPATP RCPSPGPPTP PGGCCSSHLP AREGDLGPCR KCQDSPEGGS SGPGESSEER NKADSRACPP SHHTKLKKTW LTRHSEQFEC PGGCSGKEES PATGLRALKR AGSPEVQGAS RGPAPKRPSH PFPGTGRQGA RAWQETPETI IGSKAEAEQQ EEQRGPRDGR IRLQESRLVD TSCQHHLAGV TQCQSCVQAA GEVGVLTGHS QKSRRSPLEE KQLEEEDSSA TSEEGGGGPG PEASLNKGLA KHLLSGLGDR LCRLLRKERE ALAWAQREGQ GPAMTEDSPG IPHCCSRCHH GLFNTHWRCS HCSHRLCVAC GRIAGAGKNR EKTGSQEQHT DDCAQEAGHA ACSLILTQFV SSQALAELST VMHQVWAKFD IRGHCFCQVD ARVWAPGDGG QQKEPTEKTP PTPQPSCNGD SNRTKDIKEE TPDSTESPAE DGAGRSPLPC PSLCELLAST AVKLCLGHDR IHMAFAPVTP ALPSDDRITN ILDSIIAQVV ERKIQEKALG PGLRAGSGLR KGLSLPLSPV RTRLSPPGAL LWLQEPRPKH GFHLFQEHWR QGQPVLVSGI QKTLRLSLWG MEALGTLGGQ VQTLTALGPP QPTNLDSTAF WEGFSHPETR PKLDEGSVLL LHRTLGDKDA SRVQNLASSL PLPEYCAHQG KLNLASYLPL GLTLHPLEPQ LWAAYGVNSH RGHLGTKNLC VEVSDLISIL VHAEAQLPPW YRAQKDFLSG LDGEGLWSPG SQTSTVWHVF RAQDAQRIRR FLQMVCPAGA GTLEPGAPGS CYLDAGLRRR LREEWGVSCW TLLQAPGEAV LVPAGAPHQV QGLVSTISVT QHFLSPETSA LSAQLYHQGA SLPPDHRMLY AQMDRAVFQA VKAAVGALQE AK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Hr Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Hairless (HR)

Andere Bezeichnung: Hr (HR Produkte)

Synonyme: ALUNC Protein, AU Protein, HSA277165 Protein, MUHH Protein, MUHH1 Protein, HR Protein, N Protein, ba Protein, bldy Protein, hr Protein, rh Protein, rh-bmh Protein, rhino Protein, HR, lysine demethylase and nuclear receptor corepressor Protein, hairless Protein, HR Protein, Hr Protein

Hintergrund: Histone demethylase that specifically demethylates both mono- and dimethylated 'Lys-9' of histone H3. May act as a transcription regulator controlling hair biology (via targeting of collagens), neural activity, and cell cycle (By similarity). {ECO:0000250}.

Molekulargewicht: 128.1 kDa Including tag.

UniProt: Q61645