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Myosin ID Protein (MYO1D) (AA 2-1006) (His tag)

Crystallography grade MYO1D Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3135244
  • Target Alle Myosin ID (MYO1D) Proteine anzeigen
    Myosin ID (MYO1D)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-1006
    Spezies
    • 3
    • 2
    • 2
    Maus
    Quelle
    • 4
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Myosin ID Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    AEQESLEFGK ADFVLMDTVS MPEFMANLRL RFEKGRIYTF IGEVVVSVNP YKVLNIYGRD TVEQYKGREL YERPPHLFAI ADAAYKAMKR RSKDTCIMIS GESGAGKTEA SKYIMQYIAA ITNPSQRAEI ERVKNMLLKS NCVLEAFGNA KTNRNDNSSR FGKYMDINFD FKGDPIGGHI NNYLLEKSRV IVQQPGERSF HSFYQLLQGG SEQMLHSLHL QKSLSSYNYI RVGAQLKSSI NDAAEFKVVA DAMKVIGFKP EEIQTVYKIL AVILHLGNLK FIVDGDTPLI ENGKVVSVIA ELLSTKADMV EKALLYRTVA TGRDIIDKQH TEQEASYGRD AFAKAIYERL FCWIVTRIND IIEVKNYDTT IHGKNTVIGV LDIYGFEIFD NNSFEQFCIN YCNEKLQQLF IQLVLKQEQE EYQREGIPWK HIDYFNNQII VDLVEQQHKG IIAILDDACM NVGKVTDGMF LEALNSKLGK HGHFSSRKTC ASDKILEFDR DFRIRHYAGD VVYSAIGFID KNKDTLFQDF KRLMYNSSNP VLKNMWPEGK LSITEVTKRP LTAATLFKNS MIALVDNLAS KEPYYVRCIK PNDKKSPQIF DDERCRHQVE YLGLLENVRV RRAGFAFRQT YEKFLHRYKM ISEFTWPNHD LPSDKEAVKK LIERCGFQDD VAYGKSKIFI RTPRTLFTLE ELRAQMLVRV VLFLQKVWRG TLARMRYKRT KAALTIIRYY RRYKVKSYIH EVARRFHGVK NMRDYGKHVK WPTPPKVLRR FEEALQSIFN RWRASQLIKT IPASDLPQVR AKVAAMEMLK GQRADLGLQR AWEGNYLASK PDTPQTSGTF VPVANELKRK DKYMNVLFSC HVRKVNRFSK VEDRAIFVTD RHLYKMDPTK QYKVMKTIPL YNLTGLSVSN GKDQLVVFHT KDNKDLIVCL FSKQPTHESR IGELVGVLVN HFKSEKRHLQ VNVTNPVQCS LHGKKCTVSV ETRLNQPQPD FTKNRSGFIL SVPGN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Myo1d Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MYO1D Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Myosin ID (MYO1D)
    Andere Bezeichnung
    Myo1d (MYO1D Produkte)
    Synonyme
    myr4 Protein, Myo1c Protein, Myr2 Protein, Myr4 Protein, 9930104H07Rik Protein, AW544947 Protein, D11Ertd9e Protein, myosin-1d Protein, MYO1D Protein, DKFZp469G2116 Protein, myosin ID Protein, myosin ID L homeolog Protein, MYO1D Protein, Myo1d Protein, myo1d.L Protein, myo1d Protein
    Hintergrund
    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity). {ECO:0000250|UniProtKB:P97479}.
    Molekulargewicht
    116.9 kDa Including tag.
    UniProt
    Q5SYD0
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