ELL2 Protein (AA 1-639) (Strep Tag)

Produktnummer ABIN3135064

Produktdetails

Target: ELL2 (Elongation Factor, RNA Polymerase II, 2 (ELL2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-639

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ELL2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAAGGAAGLR EEQRYGLACG RLGQDNITVL HVKLTETAIR ALETYQSHKN LIPFRPSIQF QGLQGLMKIP KNDPFNEVQN FNFYLSNVGR DNPQGSFDCI QQTLSSSGAS QLNCLGFIQD KITVCATNDS YQMTRERMTQ AEEESRNRST KVIKPGGPYV GKRVQIRKAP QAISDTVPER KRSTPMNPAN TIRKMHSGNS VSQRPYRDRV IHLLALKAYK KPELLARLQK DGVNQKDKNS LGAILQQVAN LNPKDLSYTL KDYVFKELQR DWPGYSETDR QTLDLVLSRK LNPSQNASTS RSESPLCSSK DAASSPQKRP LDSDFIDPLM NKKARISHLT NRVPPTLNGY LNPTSEKSCA GLLPPPAAAA IPTLSPLPST HLPVSNPPQT VNSNSNSPST PEGLGTQDLP VDSFSQNGSI FEDQQEKYTS RTCLETLPPS SALLKCPKPM EEEHPVSHKK SKKKSKKHKE KDQIKKLDIE TMEEKEEDLQ REETAKLSNA SPNPNEGVKE GCTASMEPSS ALELPDYLIK YIAIVSYEQR QNYKDDFNAE YDEYRALHAR METVARRFIK LDAQRKRLSP GSKEYQNVHE EVLQEYQKIK QSSPNYHEEK YRCEYLHNKL AHIKRLIGEF DQQQAESWH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ELL2 (Elongation Factor, RNA Polymerase II, 2 (ELL2))

Andere Bezeichnung: Ell2 (ELL2 Produkte)

Synonyme: cb244 Protein, fb78f03 Protein, sb:cb244 Protein, im:3814960 Protein, wu:fb78f03 Protein, xell2 Protein, MGC82755 Protein, ELL2 Protein, elongation factor for RNA polymerase II 2 Protein, elongation factor, RNA polymerase II, 2 Protein, elongation factor, RNA polymerase II 2 S homeolog Protein, elongation factor, RNA polymerase II 2 Protein, elongation factor RNA polymerase II 2 Protein, ELL2 Protein, ell2 Protein, ell2.S Protein, Ell2 Protein

Hintergrund: RNA polymerase II elongation factor ELL2,FUNCTION: Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (By similarity). Plays a role in immunoglobulin secretion in plasma cells: directs efficient alternative mRNA processing, influencing both proximal poly(A) site choice and exon skipping, as well as immunoglobulin heavy chain (IgH) alternative processing. Probably acts by regulating histone modifications accompanying transition from membrane-specific to secretory IgH mRNA expression. {ECO:0000250, ECO:0000269|PubMed:19749764, ECO:0000269|PubMed:21832080}.

Molekulargewicht: 72.1 kDa

UniProt: Q3UKU1