MAP3K9 Protein (AA 1-1077) (Strep Tag)

Produktnummer ABIN3135012

Produktdetails

Target: MAP3K9 (Mitogen-Activated Protein Kinase Kinase Kinase 9 (MAP3K9))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1077

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses MAP3K9 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MESSRSLLGC LASATAAPPG DDATGAGAEE EEDEEEAAAE LGSHAALPYW TAVFEYEAAG EDELTLRLGD VVEVLSKDSQ VSGDEGWWTG QLNQRVGIFP SNYVTPRSAF SSRCQPGAED PSCYPPIQLL EIDFAELTLE EIIGIGGFGK VYRAFWAGDE VAVKAARHDP DEDISQTIEN VRQEAKLFAM LKHPNIIALR GVCLKEPNLC LVMEFARGGP LNRVLSGKRI PPDILVNWAV QIARGMNYLH DEAIVPIIHR DLKSSNILIL QKVENGDLSN KILKITDFGL AREWHRTTKM SAAGTYAWMA PEVIRASMFS KGSDVWSYGV LLWELLTGEV PFRGIDGLAV AYGVAMNKLA LPIPSTCPEP FAKLMEDCWN PDPHSRPSFT SILDQLTTIE ESGFFEMPKD SFHCLQDDWK HEIQEMFDQL RAKEKELRTW EEELTRAALQ QKNQEELLRR REQELAEREI DILERELNII IHQLCQEKPR VKKRKGKFRK SRLKLKDGNR ISLPSDFQHK FTVQASPTMD KRKSLISNRS SPPASPTIIP RLRAIQLTPG ESSKTWGRSS VVPKEEGEEE EKRAPKKKGR TWGPGTLGQK ELTSGDEGLK SLVDGYKQWS SSAPNLGKGP RSSPALPGFT SLMEIEDEDS EGPGSGENHQ QHSPNQSYLC IPFPRGEDGD GPSSDGVHEE PTPVNSATST PQLTPTNSLK RGGTHHRRCE VALLGCGAVL AATGLGFDLL EAGKCQLLPP EEPEPPAREE KKRREGLFQR ASRPRRSTSP PSRKLFKKEE PMTLLGDPSA SLTLLSLSSI SECNSTRSLL RSDSDEIVVY EMPVSPVEAP PLTQCTHNPL VNVRVERFKR DPNQSLTPTH VTLTAPTQPS GHRRTPSDGA LKPTAAPAVL GSRSPSSNGM SPSPGTGMLK TPSPSRDPGE FPRLPDPNVV FPPTPRRWNT QRDSTLERPK TLEFLPRPRP SANRQRLDPW WFVSPSHARS ASPANSSSTE TPSNLDSCFA SSSSTVEERP GLPALLPLQA GPLLPAERTL LDLDAEGQSQ DSTVPLCRAE LNAHGPSPYE IQQEFWS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MAP3K9 (Mitogen-Activated Protein Kinase Kinase Kinase 9 (MAP3K9))

Andere Bezeichnung: Map3k9 (MAP3K9 Produkte)

Synonyme: MEKK9 Protein, MLK1 Protein, PRKE1 Protein, E130314H24Rik Protein, Mlk1 Protein, Prke1 Protein, RGD1562149 Protein, mitogen-activated protein kinase kinase kinase 9 Protein, MAP3K9 Protein, Map3k9 Protein

Hintergrund: Mitogen-activated protein kinase kinase kinase 9 (EC 2.7.11.25),FUNCTION: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Also plays a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis (By similarity). {ECO:0000250}.

Molekulargewicht: 118.8 kDa

UniProt: Q3U1V8