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CBX3 Protein (AA 2-183) (His tag)

Crystallography grade CBX3 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3133349
  • Target Alle CBX3 Proteine anzeigen
    CBX3 (Chromobox Homolog 3 (CBX3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-183
    Spezies
    • 4
    • 2
    • 1
    Maus
    Quelle
    • 4
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses CBX3 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    ASNKTTLQKM GKKQNGKSKK VEEAEPEEFV VEKVLDRRVV NGKVEYFLKW KGFTDADNTW EPEENLDCPE LIEDFLNSQK AGKEKDGTKR KSLSDSESDD SKSKKKRDAA DKPRGFARGL DPERIIGATD SSGELMFLMK WKDSDEADLV LAKEANMKCP QIVIAFYEER LTWHSCPEDE AQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cbx3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CBX3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CBX3 (Chromobox Homolog 3 (CBX3))
    Andere Bezeichnung
    Cbx3 (CBX3 Produkte)
    Synonyme
    HECH Protein, HP1-GAMMA Protein, HP1Hs-gamma Protein, Hp1-gamma Protein, HP1g Protein, M32 Protein, cbx3 Protein, cbx3a Protein, xhp1g Protein, wu:fb61b02 Protein, zgc:153397 Protein, chromobox 3 Protein, chromobox 3 L homeolog Protein, chromobox protein homolog 3 pseudogene Protein, chromobox homolog 3a (HP1 gamma homolog, Drosophila) Protein, CBX3 Protein, Cbx3 Protein, cbx3.L Protein, LOC100355282 Protein, cbx3a Protein
    Hintergrund
    Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Probably involved in the repression of many genes located in euchromatin, such as E2F1, MYC and CDC25A. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. {ECO:0000269|PubMed:24413057}.
    Molekulargewicht
    21.7 kDa Including tag.
    UniProt
    P23198
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