MYLIP Protein (AA 1-445) (His tag)
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- Target Alle MYLIP Proteine anzeigen
- MYLIP (Myosin Regulatory Light Chain Interacting Protein (MYLIP))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-445
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Spezies
- Maus
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses MYLIP Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
- Sequenz
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MLCYVTRPDA VLMEVEVEAK ANGEDCLNQV CRRLGIIEVD YFGLQFTGSK GESLWLNLRN RISQQMDGLA PYRLKLRVKF FVEPHLILQE QTRHIFFLHI KESLLAGHLQ CSPEQAVELS ALLAQTKFGD YNQNTAQYSY EDLCEKELSS STLNSIVAKH KELEGISQAS AEYQVLQIVS AMENYGIEWH AVRDSEGQKL LIGVGPEGIS ICKEDFSPIN RIAYPVVQMA TQSGKNVYLT VTKESGNSIV LLFKMISTRA ASGLYRAITE THAFYRCDTV TSAVMMQYSR DLKGHLASLF LNENINLGKK YVFDIKRTSK EVYDHARRAL YNAGVVDLVS RSDQSPPSSP LKSSDSSMSC SSCEGLSCQQ TRVLQEKLRK LKEAMLCMAC CEEEINSTFC PCGHTVCCES CAAQLQSCPV CRSRVEHVQH VYLPTHTSLL NLTVI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Mylip Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product MYLIP Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- MYLIP (Myosin Regulatory Light Chain Interacting Protein (MYLIP))
- Andere Bezeichnung
- Mylip (MYLIP Produkte)
- Synonyme
- IDOL Protein, MIR Protein, 9430057C20Rik Protein, Mir Protein, mylip Protein, wu:fj36b03 Protein, zgc:55987 Protein, MYLIP Protein, mir Protein, zgc:153767 Protein, myosin regulatory light chain interacting protein Protein, myosin regulatory light chain interacting protein a Protein, myosin regulatory light chain interacting protein L homeolog Protein, myosin regulatory light chain interacting protein b Protein, MYLIP Protein, Mylip Protein, mylipa Protein, mylip.L Protein, mylip Protein, mylipb Protein
- Hintergrund
- E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. {ECO:0000269|PubMed:19520913, ECO:0000269|PubMed:20427281}.
- Molekulargewicht
- 50.8 kDa Including tag.
- UniProt
- Q8BM54
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