TRIM17 / RNF16 Protein (TRIM17) (AA 1-477) (Strep Tag)

Produktnummer ABIN3124105

Produktdetails

Target: TRIM17 / RNF16 (TRIM17)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-477

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TRIM17 / RNF16 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MDAVELARRL QEEATCSICL DYFTDPVMTA CGHNFCRECI QMSWEKGKVK KGKKKQKGSF PCPECREMSP QRNLRPNRLL TKVAEMARQH PGLQKRDLCQ AHQEPLKLFC QDDQSPICVV CREAQEHRMH RVLPLDEAAR EYKLKLEEDI KYLREEMMKT ETLQAKEEQT LTEWQERVKE RRERILEEFQ KVVLFLVEEE RRILQVLKKE EEDTLGKLQD SKASLDHQSR SLDLILLQLE ERSQQEPLQM LQDVKDTLNR KESFSVQYPE VVLPAAIKTL CRVPGQIEVL KSFQEDVMPD PSSAYPYLLL YESRQRRYLS PPPEGSAPYS KDRFVAYPCA VGQKSFSSGR HYWEVGMNLT GDALWALGVC RDNVSRKDRV LKSPENGFWV VQLSKGKKQL SLLPNSTLVT LTEPPSHMGI FLDFQAGEVS FYSVNDGSHL HSFSQAAFPG PLLPFFCLGA PKSGQMVIST VTMWVKG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TRIM17 / RNF16 (TRIM17)

Andere Bezeichnung: Trim17 (TRIM17 Produkte)

Synonyme: TRIM17 Protein, RBCC Protein, RNF16 Protein, terf Protein, Rnf16 Protein, tripartite motif containing 17 Protein, tripartite motif-containing 17 Protein, TRIM17 Protein, Trim17 Protein

Hintergrund: E3 ubiquitin-protein ligase TRIM17 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase TRIM17) (Tripartite motif-containing protein 17),FUNCTION: E3 ubiquitin ligase that plays important roles in the regulation of neuronal apoptosis, selective autophagy or cell proliferation (PubMed:20559321, PubMed:22976837, PubMed:25215946). Stimulates the degradation of kinetochore ZW10 interacting protein ZWINT in a proteasome-dependent manner, leading to negative regulation of cell proliferation. Inhibits autophagic degradation of diverse known targets while contributing to autophagy of midbodies. Autophagy-inhibitory activity involves MCL1, which TRIM17 assembles into complexes with the key autophagy regulator BECN1 (By similarity). Controls neuronal apoptosis by mediating ubiquitination and degradation of MCL1 to initiate neuronal death (PubMed:22976837). In addition, regulates NFAT transcription factors NFATC3 and NFATC4 activities by preventing their nuclear localization, thus inhibiting their transcriptional activities (PubMed:25215946). Decreases TRIM41-mediated degradation of ZSCAN2 thereby stimulating alpha-synuclein/SNCA transcription in neuronal cells (PubMed:30485814). Prevents the E3 ubiquitin-ligase activity of TRIM28 and its interaction with anti-apoptotic BCL2A1, blocking TRIM28 from ubiquitinating BCL2A1 (By similarity). {ECO:0000250|UniProtKB:Q9Y577, ECO:0000269|PubMed:20559321, ECO:0000269|PubMed:22976837, ECO:0000269|PubMed:25215946, ECO:0000269|PubMed:30485814}.

Molekulargewicht: 54.9 kDa

UniProt: Q7TPM3