Aspartyl Aminopeptidase Protein (DNPEP) (AA 1-473) (Strep Tag)

Produktnummer ABIN3120411

Produktdetails

Target: Aspartyl Aminopeptidase (DNPEP)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-473

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Aspartyl Aminopeptidase Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAMNGRARKE AIQATARELL KFVNRSPSPF HVVAECRSRL LQAGFRELKE TEGWDIVPEN KYFLTRNSSS IIAFAVGGQY VPGNGFSLIG AHTDSPCLRV KRKSRRSQVG YHQVGVETYG GGIWSTWFDR DLTLAGRVII KCPTSGRLEQ RLVHIERPIL RIPHLAIHLQ RNINENFGPN TEIHLVPILA TAVQEELEKG TPEPGPLGAT DERHHSVLMS LLCTHLGLSP DSIMEMELCL ADTQPAVLGG AYEEFIFAPR LDNLHSCFCA LQALIDSCAS PASLARDPHV RMVTLYDNEE VGSESAQGAQ SLLTELILRR ISASPQRLTA FEEAIPKSFM ISADMAHAVH PNYSDKHEEN HRPLFHKGPV IKVNSKQRYA SNAVSESMIR EVAGQVGVPL QDLMVRNDSP CGTTIGPILA SRLGLRVLDL GSPQLAMHSI RETACTTGVL QTLTLFKGFF ELFPSVSRNL LVD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Aspartyl Aminopeptidase (DNPEP)

Andere Bezeichnung: Dnpep (DNPEP Produkte)

Synonyme: zgc:55944 Protein, MGC80319 Protein, PSPTO3876 Protein, DDBDRAFT_0186837 Protein, DDBDRAFT_0266791 Protein, DDB_0186837 Protein, DDB_0266791 Protein, dnpep Protein, ASPEP Protein, DAP Protein, AA407814 Protein, aspartyl aminopeptidase Protein, aspartyl aminopeptidase L homeolog Protein, Aspartyl aminopeptidase Protein, dnpep Protein, DNPEP Protein, dnpep.L Protein, PSPTO_3876 Protein, DNP1 Protein, LbPR_M2 Protein, Arch_1448 Protein, Saut_1010 Protein, Ilyop_2166 Protein, Sulku_0925 Protein, TSTA_003400 Protein, Intca_2758 Protein, Despr_2295 Protein, Odosp_2771 Protein, Selsp_1665 Protein, Dnpep Protein

Hintergrund: Aspartyl aminopeptidase (EC 3.4.11.21),FUNCTION: Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism (By similarity). {ECO:0000250}.

Molekulargewicht: 52.2 kDa

UniProt: Q9Z2W0