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TSG101 Protein (AA 2-390) (His tag)

Crystallography grade TSG101 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3096136
  • Target Alle TSG101 Proteine anzeigen
    TSG101 (Tumor Susceptibility Gene 101 (TSG101))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-390
    Spezies
    • 5
    • 1
    Human
    Quelle
    • 3
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses TSG101 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    AVSESQLKKM VSKYKYRDLT VRETVNVITL YKDLKPVLDS YVFNDGSSRE LMNLTGTIPV PYRGNTYNIP ICLWLLDTYP YNPPICFVKP TSSMTIKTGK HVDANGKIYL PYLHEWKHPQ SDLLGLIQVM IVVFGDEPPV FSRPISASYP PYQATGPPNT SYMPGMPGGI SPYPSGYPPN PSGYPGCPYP PGGPYPATTS SQYPSQPPVT TVGPSRDGTI SEDTIRASLI SAVSDKLRWR MKEEMDRAQA ELNALKRTEE DLKKGHQKLE EMVTRLDQEV AEVDKNIELL KKKDEELSSA LEKMENQSEN NDIDEVIIPT APLYKQILNL YAEENAIEDT IFYLGEALRR GVIDLDVFLK HVRLLSRKQF QLRALMQKAR KTAGLSDLY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TSG101 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product TSG101 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    TSG101 (Tumor Susceptibility Gene 101 (TSG101))
    Andere Bezeichnung
    TSG101 (TSG101 Produkte)
    Synonyme
    CG9712 Protein, DmTSG101 Protein, Dmel\\CG9712 Protein, ESCRT-I Protein, Tsg101 Protein, burs Protein, dTSg101 Protein, dTsg101 Protein, dVps23 Protein, ept Protein, tsg101 Protein, vps23 Protein, TSG101 Protein, MGC76193 Protein, TSG10 Protein, VPS23 Protein, AI255943 Protein, CC2 Protein, Rw Protein, zgc:86926 Protein, tumor susceptibility 101 Protein, Tumor susceptibility gene 101 Protein, Tumor Susceptibility Gene homolog Protein, tumor susceptibility gene 101 Protein, tumor susceptibility 101a Protein, tumor susceptibility 101 L homeolog Protein, TSG101 Protein, tsg-101 Protein, tsg101 Protein, Tsg101 Protein, tsg101a Protein, tsg101.L Protein
    Hintergrund
    Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis, the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:11916981, ECO:0000269|PubMed:17556548, ECO:0000269|PubMed:17853893, ECO:0000269|PubMed:21070952, ECO:0000269|PubMed:21757351, ECO:0000269|PubMed:22660413}.
    Molekulargewicht
    44.8 kDa Including tag.
    UniProt
    Q99816
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