Sphingomyelin Synthase 2 (SGMS2) (AA 1-365) protein (rho-1D4 tag)

Details zu Produkt Nr. ABIN3095466, Anbieter: Anmelden zum Anzeigen
Proteinname
  • SMS2
  • 4933405A16Rik
  • 5133401H06Rik
  • AI854299
  • RGD1305778
  • spermatin
  • sphingomyelin synthase 2
  • sgms2
  • SGMS2
  • Sgms2
Proteineigenschaft
AA 1-365
2
1
1
1
1
Spezies
Human
3
1
Quelle
Insektenzellen
2
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses Sphingomyelin Synthase 2 Protein ist gelabelt mit rho-1D4 tag.
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz MDIIETAKLE EHLENQPSDP TNTYARPAEP VEEENKNGNG KPKSLSSGLR KGTKKYPDYI QIAMPTESRN KFPLEWWKTG IAFIYAVFNL VLTTVMITVV HERVPPKELS PPLPDKFFDY IDRVKWAFSV SEINGIILVG LWITQWLFLR YKSIVGRRFC FIIGTLYLYR CITMYVTTLP VPGMHFQCAP KLNGDSQAKV QRILRLISGG GLSITGSHIL CGDFLFSGHT VTLTLTYLFI KEYSPRHFWW YHLICWLLSA AGIICILVAH EHYTIDVIIA YYITTRLFWW YHSMANEKNL KVSSQTNFLS RAWWFPIFYF FEKNVQGSIP CCFSWPLSWP PGCFKSSCKK YSRVQKIGED NEKST
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human SGMS2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Reinigung Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin-free.
Güteklasse Crystallography grade
Hintergrund Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. {ECO:0000269|PubMed:14685263, ECO:0000269|PubMed:17449912}.
Molekulargewicht 43.5 kDa Including tag.
UniProt Q8NHU3
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Sphingomyelin Synthase 2 (SGMS2) (AA 1-365) protein (rho-1D4 tag) (ABIN3095466) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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