RAB1A Protein (AA 2-205) (His tag)

Produktnummer ABIN3094907

Produktdetails

Target: RAB1A (RAB1A, Member RAS Oncogene Family (RAB1A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-205

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses RAB1A Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: SSMNPEYDYL FKLLLIGDSG VGKSCLLLRF ADDTYTESYI STIGVDFKIR TIELDGKTIK LQIWDTAGQE RFRTITSSYY RGAHGIIVVY DVTDQESFNN VKQWLQEIDR YASENVNKLL VGNKCDLTTK KVVDYTTAKE FADSLGIPFL ETSAKNATNV EQSFMTMAAE IKKRMGPGAT AGGAEKSNVK IQSTPVKQSG GGCC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RAB1A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RAB1A (RAB1A, Member RAS Oncogene Family (RAB1A))

Andere Bezeichnung: RAB1A (RAB1A Produkte)

Synonyme: RAB1 Protein, YPT1 Protein, RAS Protein, RAB1B Protein, fb53a02 Protein, oncogene Protein, wu:fb53a02 Protein, si:zc101n13.3 Protein, rab1 Protein, MGC53138 Protein, RAB1A Protein, AAF55873 Protein, CG3320 Protein, DRAB1 Protein, DRab1 Protein, Dm Rab1 Protein, DmRab1 Protein, Dmel\\CG3320 Protein, RAB1a Protein, Rab1a Protein, dRab1 Protein, dar6 Protein, drab1 Protein, DDBDRAFT_0185730 Protein, DDBDRAFT_0191476 Protein, DDB_0185730 Protein, DDB_0191476 Protein, Gtbp Protein, Rab-1 Protein, Rab1A Protein, Ypt1 Protein, mKIAA3012 Protein, Ac2-048 Protein, Rab1 Protein, Rab1r Protein, RAB1A, member RAS oncogene family Protein, RAB1A, member RAS oncogene family b Protein, RAB1A, member RAS oncogene family L homeolog Protein, RAB1B, member RAS oncogene family Protein, CG3320 gene product from transcript CG3320-RA Protein, rab1A protein Protein, PfRab1a Protein, hypothetical protein Protein, Rab GTPase Protein, angiogenin Protein, RAB1A Protein, rab1ab Protein, rab1a.L Protein, RAB1B Protein, rab1a Protein, Rab1 Protein, rab1A Protein, Rab1a Protein, ANG Protein

Hintergrund: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport. {ECO:0000269|PubMed:20639577, ECO:0000269|PubMed:20861236, ECO:0000269|PubMed:21303926, ECO:0000269|PubMed:22939626}.

Molekulargewicht: 23.5 kDa Including tag.

UniProt: P62820

Pathways: SARS-CoV-2 Protein Interaktom