POGZ Protein (AA 1-1410) (Strep Tag)

Produktnummer ABIN3094734

Produktdetails

Target: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1410

Spezies: Human

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses POGZ Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MADTDLFMEC EEEELEPWQK ISDVIEDSVV EDYNSVDKTT TVSVSQQPVS APVPIAAHAS VAGHLSTSTT VSSSGAQNSD STKKTLVTLI ANNNAGNPLV QQGGQPLILT QNPAPGLGTM VTQPVLRPVQ VMQNANHVTS SPVASQPIFI TTQGFPVRNV RPVQNAMNQV GIVLNVQQGQ TVRPITLVPA PGTQFVKPTV GVPQVFSQMT PVRPGSTMPV RPTTNTFTTV IPATLTIRST VPQSQSQQTK STPSTSTTPT ATQPTSLGQL AVQSPGQSNQ TTNPKLAPSF PSPPAVSIAS FVTVKRPGVT GENSNEVAKL VNTLNTIPSL GQSPGPVVVS NNSSAHGSQR TSGPESSMKV TSSIPVFDLQ DGGRKICPRC NAQFRVTEAL RGHMCYCCPE MVEYQKKGKS LDSEPSVPSA AKPPSPEKTA PVASTPSSTP IPALSPPTKV PEPNENVGDA VQTKLIMLVD DFYYGRDGGK VAQLTNFPKV ATSFRCPHCT KRLKNNIRFM NHMKHHVELD QQNGEVDGHT ICQHCYRQFS TPFQLQCHLE NVHSPYESTT KCKICEWAFE SEPLFLQHMK DTHKPGEMPY VCQVCQYRSS LYSEVDVHFR MIHEDTRHLL CPYCLKVFKN GNAFQQHYMR HQKRNVYHCN KCRLQFLFAK DKIEHKLQHH KTFRKPKQLE GLKPGTKVTI RASRGQPRTV PVSSNDTPPS ALQEAAPLTS SMDPLPVFLY PPVQRSIQKR AVRKMSVMGR QTCLECSFEI PDFPNHFPTY VHCSLCRYST CCSRAYANHM INNHVPRKSP KYLALFKNSV SGIKLACTSC TFVTSVGDAM AKHLVFNPSH RSSSILPRGL TWIAHSRHGQ TRDRVHDRNV KNMYPPPSFP TNKAATVKSA GATPAEPEEL LTPLAPALPS PASTATPPPT PTHPQALALP PLATEGAECL NVDDQDEGSP VTQEPELASG GGGSGGVGKK EQLSVKKLRV VLFALCCNTE QAAEHFRNPQ RRIRRWLRRF QASQGENLEG KYLSFEAEEK LAEWVLTQRE QQLPVNEETL FQKATKIGRS LEGGFKISYE WAVRFMLRHH LTPHARRAVA HTLPKDVAEN AGLFIDFVQR QIHNQDLPLS MIVAIDEISL FLDTEVLSSD DRKENALQTV GTGEPWCDVV LAILADGTVL PTLVFYRGQM DQPANMPDSI LLEAKESGYS DDEIMELWST RVWQKHTACQ RSKGMLVMDC HRTHLSEEVL AMLSASSTLP AVVPAGCSSK IQPLDVCIKR TVKNFLHKKW KEQAREMADT ACDSDVLLQL VLVWLGEVLG VIGDCPELVQ RSFLVASVLP GPDGNINSPT RNADMQEELI ASLEEQLKLS GEHSESSTPR PRSSPEETIE PESLHQLFEG ESETESFYGF EEADLDLMEI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: POGZ (Pogo Transposable Element with ZNF Domain (POGZ))

Andere Bezeichnung: POGZ (POGZ Produkte)

Synonyme: POGZ Protein, ZNF280E Protein, ZNF635 Protein, ZNF635m Protein, 9530006B08Rik Protein, AU015913 Protein, AU044539 Protein, pogo transposable element with ZNF domain Protein, pogo transposable element derived with ZNF domain Protein, POGZ Protein, pogz Protein, Pogz Protein

Hintergrund: Pogo transposable element with ZNF domain (Suppressor of hairy wing homolog 5) (Zinc finger protein 280E) (Zinc finger protein 635),FUNCTION: Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PubMed:20562864). Promotes the repair of DNA double-strand breaks through the homologous recombination pathway (PubMed:26721387). {ECO:0000269|PubMed:20562864, ECO:0000269|PubMed:26721387}.

Molekulargewicht: 155.3 kDa

UniProt: Q7Z3K3