PLTP Protein (AA 18-493) (His tag)

Produktnummer ABIN3094644

Produktdetails

Target: PLTP (Phospholipid Transfer Protein (PLTP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 18-493

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PLTP Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: EFPGCKIRVT SKALELVKQE GLRFLEQELE TITIPDLRGK EGHFYYNISE VKVTELQLTS SELDFQPQQE LMLQITNASL GLRFRRQLLY WFFYDGGYIN ASAEGVSIRT GLELSRDPAG RMKVSNVSCQ ASVSRMHAAF GGTFKKVYDF LSTFITSGMR FLLNQQICPV LYHAGTVLLN SLLDTVPVRS SVDELVGIDY SLMKDPVAST SNLDMDFRGA FFPLTERNWS LPNRAVEPQL QEEERMVYVA FSEFFFDSAM ESYFRAGALQ LLLVGDKVPH DLDMLLRATY FGSIVLLSPA VIDSPLKLEL RVLAPPRCTI KPSGTTISVT ASVTIALVPP DQPEVQLSSM TMDARLSAKM ALRGKALRTQ LDLRRFRIYS NHSALESLAL IPLQAPLKTM LQIGVMPMLN ERTWRGVQIP LPEGINFVHE VVTNHAGFLT IGADLHFAKG LREVIEKNRP ADVRASTAPT PSTAAV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PLTP Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PLTP (Phospholipid Transfer Protein (PLTP))

Andere Bezeichnung: PLTP (PLTP Produkte)

Synonyme: zgc:100903 Protein, MGC84445 Protein, PLTP Protein, hdlcq9 Protein, DKFZp469A1437 Protein, BPIFE Protein, HDLCQ9 Protein, Bpife Protein, OD107 Protein, phospholipid transfer protein Protein, phospholipid transfer protein S homeolog Protein, PLTP Protein, pltp Protein, pltp.S Protein, LOC542343 Protein, Pltp Protein

Hintergrund: Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability.

Molekulargewicht: 54.0 kDa Including tag.

UniProt: P55058

Pathways: Lipid Metabolism