PKN1 Protein (AA 2-942) (His tag)

Produktnummer ABIN3094604

Produktdetails

Target: PKN1 (Protein Kinase N1 (PKN1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-942

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PKN1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: ASDAVQSEPR SWSLLEQLGL AGADLAAPGV QQQLELERER LRREIRKELK LKEGAENLRR ATTDLGRSLG PVELLLRGSS RRLDLLHQQL QELHAHVVLP DPAATHDGPQ SPGAGGPTCS ATNLSRVAGL EKQLAIELKV KQGAENMIQT YSNGSTKDRK LLLTAQQMLQ DSKTKIDIIR MQLRRALQAG QLENQAAPDD TQGSPDLGAV ELRIEELRHH FRVEHAVAEG AKNVLRLLSA AKAPDRKAVS EAQEKLTESN QKLGLLREAL ERRLGELPAD HPKGRLLREE LAAASSAAFS TRLAGPFPAT HYSTLCKPAP LTGTLEVRVV GCRDLPETIP WNPTPSMGGP GTPDSRPPFL SRPARGLYSR SGSLSGRSSL KAEAENTSEV STVLKLDNTV VGQTSWKPCG PNAWDQSFTL ELERARELEL AVFWRDQRGL CALKFLKLED FLDNERHEVQ LDMEPQGCLV AEVTFRNPVI ERIPRLRRQK KIFSKQQGKA FQRARQMNID VATWVRLLRR LIPNATGTGT FSPGASPGSE ARTTGDISVE KLNLGTDSDS SPQKSSRDPP SSPSSLSSPI QESTAPELPS ETQETPGPAL CSPLRKSPLT LEDFKFLAVL GRGHFGKVLL SEFRPSGELF AIKALKKGDI VARDEVESLM CEKRILAAVT SAGHPFLVNL FGCFQTPEHV CFVMEYSAGG DLMLHIHSDV FSEPRAIFYS ACVVLGLQFL HEHKIVYRDL KLDNLLLDTE GYVKIADFGL CKEGMGYGDR TSTFCGTPEF LAPEVLTDTS YTRAVDWWGL GVLLYEMLVG ESPFPGDDEE EVFDSIVNDE VRYPRFLSAE AIGIMRRLLR RNPERRLGSS ERDAEDVKKQ PFFRTLGWEA LLARRLPPPF VPTLSGRTDV SNFDEEFTGE APTLSPPRDA RPLTAAEQAA FLDFDFVAGG C
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PKN1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PKN1 (Protein Kinase N1 (PKN1))

Andere Bezeichnung: PKN1 (PKN1 Produkte)

Synonyme: DBK Protein, PAK-1 Protein, PAK1 Protein, PKN Protein, PKN-ALPHA Protein, PRK1 Protein, PRKCL1 Protein, F730027O18Rik Protein, Pkn Protein, Prkcl1 Protein, Stk3 Protein, PKN1 Protein, 3-2 Protein, CG2049 Protein, CG2055 Protein, CT6637 Protein, CT6660 Protein, DPKN Protein, Dmel\\CG2049 Protein, Dpkn Protein, PRK2 Protein, Pk45C Protein, Pk?3 Protein, dPKN Protein, l(2)06736 Protein, l(2)45Ca Protein, v(2)rG232 Protein, protein kinase N1 Protein, protein kinase N1b Protein, Protein kinase N Protein, PKN1 Protein, Pkn1 Protein, pkn1b Protein, pkn1 Protein, Pkn Protein

Hintergrund: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro. {ECO:0000269|PubMed:11104762, ECO:0000269|PubMed:12514133, ECO:0000269|PubMed:17332740, ECO:0000269|PubMed:18066052, ECO:0000269|PubMed:20188095, ECO:0000269|PubMed:21224381, ECO:0000269|PubMed:21754995, ECO:0000269|PubMed:24248594, ECO:0000269|PubMed:8557118, ECO:0000269|PubMed:8621664, ECO:0000269|PubMed:9175763}.

Molekulargewicht: 104.8 kDa Including tag.

UniProt: Q16512