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NPC1 Protein (AA 854-1098) (His tag)

Crystallography grade NPC1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Produktnummer ABIN3094185
  • Target Alle NPC1 Proteine anzeigen
    NPC1 (Niemann-Pick Disease, Type C1 (NPC1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 854-1098
    Spezies
    • 6
    • 5
    • 1
    Human
    Quelle
    • 5
    • 3
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses NPC1 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    NKVDIGLDQS LSMPDDSYMV DYFKSISQYL HAGPPVYFVL EEGHDYTSSK GQNMVCGGMG CNNDSLVQQI FNAAQLDNYT RIGFAPSSWI DDYFDWVKPQ SSCCRVDNIT DQFCNASVVD PACVRCRPLT PEGKQRPQGG DFMRFLPMFL SDNPNPKCGK GGHAAYSSAV NILLGHGTRV GATYFMTYHT VLQTSADFID ALKKARLIAS NVTETMGING SAYRVFPYSV FYVFYEQYLT IIDDT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human NPC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product NPC1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    NPC1 (Niemann-Pick Disease, Type C1 (NPC1))
    Andere Bezeichnung
    NPC1 (NPC1 Produkte)
    Synonyme
    NPC Protein, 9130221N23Rik Protein, Gm243 Protein, Cdig2 Protein, im:7149020 Protein, wu:fb53a12 Protein, wu:fc29a12 Protein, A430089E03Rik Protein, C85354 Protein, D18Ertd139e Protein, D18Ertd723e Protein, lcsd Protein, nmf164 Protein, spm Protein, NPC intracellular cholesterol transporter 1 Protein, NPC1 like intracellular cholesterol transporter 1 Protein, Niemann-Pick disease, type C1 Protein, Niemann-Pick C1 protein Protein, NPC1 Protein, Npc1l1 Protein, Npc1 Protein, npc1 Protein, LOC579887 Protein
    Hintergrund
    Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. {ECO:0000269|PubMed:18772377, ECO:0000269|PubMed:19563754}., (Microbial infection) Acts as an endosomal entry receptor for ebolavirus. {ECO:0000269|PubMed:21866103, ECO:0000269|PubMed:25855742}.
    Molekulargewicht
    28.2 kDa Including tag.
    UniProt
    O15118
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