LCT Protein (AA 869-1927) (rho-1D4 tag)

Produktnummer ABIN3093557

Produktdetails

Target: LCT (Lactase (LCT))

Protein-Typ: Recombinant

Proteineigenschaft: AA 869-1927

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses LCT Protein ist gelabelt mit rho-1D4 tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: AFTFPSEVPS KAKVVWEKFS SQPKFERDLF YHGTFRDDFL WGVSSSAYQI EGAWDADGKG PSIWDNFTHT PGSNVKDNAT GDIACDSYHQ LDADLNMLRA LKVKAYRFSI SWSRIFPTGR NSSINSHGVD YYNRLINGLV ASNIFPMVTL FHWDLPQALQ DIGGWENPAL IDLFDSYADF CFQTFGDRVK FWMTFNEPMY LAWLGYGSGE FPPGVKDPGW APYRIAHAVI KAHARVYHTY DEKYRQEQKG VISLSLSTHW AEPKSPGVPR DVEAADRMLQ FSLGWFAHPI FRNGDYPDTM KWKVGNRSEL QHLATSRLPS FTEEEKRFIR ATADVFCLNT YYSRIVQHKT PRLNPPSYED DQEMAEEEDP SWPSTAMNRA APWGTRRLLN WIKEEYGDIP IYITENGVGL TNPNTEDTDR IFYHKTYINE ALKAYRLDGI DLRGYVAWSL MDNFEWLNGY TVKFGLYHVD FNNTNRPRTA RASARYYTEV ITNNGMPLAR EDEFLYGRFP EGFIWSAASA AYQIEGAWRA DGKGLSIWDT FSHTPLRVEN DAIGDVACDS YHKIAEDLVT LQNLGVSHYR FSISWSRILP DGTTRYINEA GLNYYVRLID TLLAASIQPQ VTIYHWDLPQ TLQDVGGWEN ETIVQRFKEY ADVLFQRLGD KVKFWITLNE PFVIAYQGYG YGTAAPGVSN RPGTAPYIVG HNLIKAHAEA WHLYNDVYRA SQGGVISITI SSDWAEPRDP SNQEDVEAAR RYVQFMGGWF AHPIFKNGDY NEVMKTRIRD RSLAAGLNKS RLPEFTESEK RRINGTYDFF GFNHYTTVLA YNLNYATAIS SFDADRGVAS IADRSWPDSG SFWLKMTPFG FRRILNWLKE EYNDPPIYVT ENGVSQREET DLNDTARIYY LRTYINEALK AVQDKVDLRG YTVWSAMDNF EWATGFSERF GLHFVNYSDP SLPRIPKASA KFYASVVRCN GFPDPATGPH ACLHQPDAGP TISPVRQEEV QFLGLMLGTT EAQTALYVLF SLVLLGVCGL AFLSYKYCKR SKQGKTQRSQ QELSPVSSF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LCT Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: LCT (Lactase (LCT))

Andere Bezeichnung: LCT (LCT Produkte)

Synonyme: LAC Protein, LPH Protein, LPH1 Protein, Lph Protein, Gm100 Protein, Lphl Protein, LCT Protein, PNGH Protein, GLH1 Protein, lac Protein, lct Protein, lph Protein, lph1 Protein, lactase Protein, lactase Protein, lactase, gene 1 L homeolog Protein, lactase, gene 2 L homeolog Protein, lactase, gene 2 Protein, lactase phlorizin hydrolase Protein, LCT Protein, Lct Protein, lct.1.L Protein, lct.2.L Protein, lct.2 Protein, LPH Protein

Hintergrund: LPH splits lactose in the small intestine.

Molekulargewicht: 122.2 kDa Including tag.

UniProt: P09848