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ANO1 Protein (AA 628-731) (His tag)

Crystallography grade ANO1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, Crys, ELISA
Produktnummer ABIN3088972
  • Target Alle ANO1 Proteine anzeigen
    ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 628-731
    Spezies
    • 4
    • 3
    Human
    Quelle
    • 5
    • 2
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses ANO1 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), ELISA
    Sequenz
    FKGRFVGRPG DYVYIFRSFR MEECAPGGCL MELCIQLSII MLGKQLIQNN LFEIGIPKMK KLIRYLKLKQ QSPPDHEECV KRKQRYEVDY NLEPFAGLTP EYME
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ANO1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ANO1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))
    Andere Bezeichnung
    ANO1 (ANO1 Produkte)
    Synonyme
    DOG1 Protein, ORAOV2 Protein, TAOS2 Protein, TMEM16A Protein, Tmem16a Protein, anoctamin 1 Protein, anoctamin 1, calcium activated chloride channel Protein, Ano1 Protein, ANO1 Protein
    Hintergrund
    Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. {ECO:0000269|PubMed:20056604, ECO:0000269|PubMed:21984732, ECO:0000269|PubMed:22178883, ECO:0000269|PubMed:22946059}.
    Molekulargewicht
    13.2 kDa Including tag.
    UniProt
    Q5XXA6
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