ANKFY1 Protein (AA 2-1169) (His tag)

Produktnummer ABIN3088741

Produktdetails

Target: ANKFY1 (Ankyrin Repeat and FYVE Domain Containing 1 (ANKFY1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-1169

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses ANKFY1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequenz: AEEEVAKLEK HLMLLRQEYV KLQKKLAETE KRCALLAAQA NKESSSESFI SRLLAIVADL YEQEQYSDLK IKVGDRHISA HKFVLAARSD SWSLANLSST KELDLSDANP EVTMTMLRWI YTDELEFRED DVFLTELMKL ANRFQLQLLR ERCEKGVMSL VNVRNCIRFY QTAEELNAST LMNYCAEIIA SHWDDLRKED FSSMSAQLLY KMIKSKTEYP LHKAIKVERE DVVFLYLIEM DSQLPGKLNE ADHNGDLALD LALSRRLESI ATTLVSHKAD VDMVDKSGWS LLHKGIQRGD LFAATFLIKN GAFVNAATLG AQETPLHLVA LYSSKKHSAD VMSEMAQIAE ALLQAGANPN MQDSKGRTPL HVSIMAGNEY VFSQLLQCKQ LDLELKDHEG STALWLAVQH ITVSSDQSVN PFEDVPVVNG TSFDENSFAA RLIQRGSHTD APDTATGNCL LQRAAGAGNE AAALFLATNG AHVNHRNKWG ETPLHTACRH GLANLTAELL QQGANPNLQT EEALPLPKEA ASLTSLADSV HLQTPLHMAI AYNHPDVVSV ILEQKANALH ATNNLQIIPD FSLKDSRDQT VLGLALWTGM HTIAAQLLGS GAAINDTMSD GQTLLHMAIQ RQDSKSALFL LEHQADINVR TQDGETALQL AIRNQLPLVV DAICTRGADM SVPDEKGNPP LWLALANNLE DIASTLVRHG CDATCWGPGP GGCLQTLLHR AIDENNEPTA CFLIRSGCDV NSPRQPGANG EGEEEARDGQ TPLHLAASWG LEETVQCLLE FGANVNAQDA EGRTPIHVAI SSQHGVIIQL LVSHPDIHLN VRDRQGLTPF ACAMTFKNNK SAEAILKRES GAAEQVDNKG RNFLHVAVQN SDIESVLFLI SVHANVNSRV QDASKLTPLH LAVQAGSEII VRNLLLAGAK VNELTKHRQT ALHLAAQQDL PTICSVLLEN GVDFAAVDEN GNNALHLAVM HGRLNNIRVL LTECTVDAEA FNLRGQSPLH ILGQYGKENA AAIFDLFLEC MPGYPLDKPD ADGSTVLLLA YMKGNANLCR AIVRSGARLG VNNNQGVNIF NYQVATKQLL FRLLDMLSKE PPWCDGSYCY ECTARFGVTT RKHHCRHCGR LLCHKCSTKE IPIIKFDLNK PVRVCNICFD VLTLGGVS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ANKFY1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ANKFY1 (Ankyrin Repeat and FYVE Domain Containing 1 (ANKFY1))

Andere Bezeichnung: ANKFY1 (ANKFY1 Produkte)

Synonyme: ANKHZN Protein, BTBD23 Protein, ZFYVE14 Protein, Ankhzn Protein, mKIAA1255 Protein, ankyrin repeat and FYVE domain containing 1 Protein, ANKFY1 Protein, Ankfy1 Protein

Hintergrund: Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extend in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Involved in macropinocytosis, the function is dependent on Rab5-GTP. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1-dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport. {ECO:0000269|PubMed:15328530, ECO:0000269|PubMed:22284051, ECO:0000269|PubMed:24102721}.

Molekulargewicht: 129.2 kDa Including tag.

UniProt: Q9P2R3