Chromosome 10 Open Reading Frame 33 (C10ORF33) (AA 1-581) protein (His tag)

Details zu Produkt Nr. ABIN3086816, Anbieter: Anmelden zum Anzeigen
Proteinname
  • C10orf33
  • DKFZp469H0233
  • FP3420
  • C26H10orf33
  • 3830409H07Rik
  • 4833409A17Rik
  • RGD1303232
  • pyridine nucleotide-disulphide oxidoreductase domain 2
  • chromosome 10 open reading frame 33
  • PYROXD2
  • Pyroxd2
Proteineigenschaft
AA 1-581
2
Spezies
Human
1
1
Quelle
Insektenzellen
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
His tag
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Sequenz MAASGRGLCK AVAASPFPAW RRDNTEARGG LKPEYDAVVI GAGHNGLVAA AYLQRLGVNT AVFERRHVIG GAAVTEEIIP GFKFSRASYL LSLLRPQIYT DLELKKHGLR LHLRNPYSFT PMLEEGAGSK VPRCLLLGTD MAENQKQIAQ FSQKDAQVFP KYEEFMHRLA LAIDPLLDAA PVDMAAFQHG SLLQRMRSLS TLKPLLKAGR ILGAQLPRYY EVLTAPITKV LDQWFESEPL KATLATDAVI GAMTSPHTPG SGYVLLHHVM GGLEGMQGAW GYVQGGMGAL SDAIASSATT HGASIFTEKT VAKVQVNSEG CVQGVVLEDG TEVRSKMVLS NTSPQITFLK LTPQEWLPEE FLERISQLDT RSPVTKINVA VDRLPSFLAA PNAPRGQPLP HHQCSIHLNC EDTLLLHQAF EDAMDGLPSH RPVIELCIPS SLDPTLAPPG CHVVSLFTQY MPYTLAGGKA WDEQERDAYA DRVFDCIEVY APGFKDSVVG RDILTPPDLE RIFGLPGGNI FHCAMSLDQL YFARPVPLHS GYRCPLQGLY LCGSGAHPGG GVMGAAGRNA AHVAFRDLKS M
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human PYROXD2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Reinigung Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin free.
Güteklasse Crystallography grade
Hintergrund Probable oxidoreductase. {ECO:0000250}.
Molekulargewicht 64.0 kDa Including tag.
UniProt Q8N2H3
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Kommentare

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Chromosome 10 Open Reading Frame 33 (C10ORF33) (AA 1-581) protein (His tag) (ABIN3086816) „Crystallography Grade“ protein due to multi-step, protein-specific purification process