PABPN1 Protein (AA 2-306) (His tag)

Produktnummer ABIN3085688

Produktdetails

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-306

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PABPN1 Protein ist gelabelt mit His tag.

Applikation: ELISA, SDS-PAGE (SDS), Crystallization (Crys), Western Blotting (WB)

Sequenz: AAAAAAAAAA GAAGGRGSGP GRRRHLVPGA GGEAGEGAPG GAGDYGNGLE SEELEPEELL LEPEPEPEPE EEPPRPRAPP GAPGPGPGSG APGSQEEEEE PGLVEGDPGD GAIEDPELEA IKARVREMEE EAEKLKELQN EVEKQMNMSP PPGNAGPVIM SIEEKMEADA RSIYVGNVDY GATAEELEAH FHGCGSVNRV TILCDKFSGH PKGFAYIEFS DKESVRTSLA LDESLFRGRQ IKVIPKRTNR PGISTTDRGF PRARYRARTT NYNSSRSRFY SGFNSRPRGR VYRGRARATS WYSPY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PABPN1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Andere Bezeichnung: PABPN1 (PABPN1 Produkte)

Synonyme: OPMD Protein, PAB2 Protein, PABII Protein, PABP-2 Protein, PABP2 Protein, Pabp3 Protein, mPABII Protein, ARABIDOPSIS POLY(A) BINDING 2 Protein, ATPAB2 Protein, F28A23.130 Protein, F28A23_130 Protein, POLY(A) BINDING PROTEIN 2 Protein, POLY(A)-BINDING PROTEIN 2 Protein, poly(A) binding protein 2 Protein, opmd Protein, pab2 Protein, pabp2 Protein, pabpii Protein, pabpn1 Protein, Pabpn1 Protein, Poly(A)-binding protein 2 Protein, fb19c03 Protein, wu:fb17e03 Protein, wu:fb18c06 Protein, wu:fb19c03 Protein, zgc:85979 Protein, zgc:92452 Protein, poly(A) binding protein nuclear 1 Protein, poly(A) binding protein, nuclear 1 Protein, poly(A) binding protein 2 Protein, poly(A) binding protein, nuclear 1 S homeolog Protein, poly(A) binding protein, nuclear 1 L homeolog Protein, polyadenylate-binding protein 2 Protein, poly(A) binding protein Pab2 Protein, PABPN1 Protein, Pabpn1 Protein, PAB2 Protein, pabpn1.S Protein, pabpn1.L Protein, LOC100728725 Protein, pab2 Protein, pabpn1 Protein

Hintergrund: Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation (By similarity). {ECO:0000250, ECO:0000269|PubMed:11371506, ECO:0000269|PubMed:11689481}.

Molekulargewicht: 33.6 kDa Including tag.

UniProt: Q86U42