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TP53I13 Protein (AA 28-309) (His tag)

Crystallography grade TP53I13 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
Produktnummer ABIN3085247
  • Target Alle TP53I13 Proteine anzeigen
    TP53I13 (Tumor Protein P53 Inducible Protein 13 (TP53I13))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 28-309
    Spezies
    • 2
    • 1
    • 1
    Human
    Quelle
    • 3
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses TP53I13 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    GPAEEAGAHC PESLWPLPPQ VSPRVTYTRV SPGQAEDVTF LYHPCAHPWL KLQLALLAYA CMANPSLTPD FSLTQDRPLV LTAWGLALEM AWVEPAWAAH WLMRRRRRKQ RKKKAWIYCE SLSGPAPSEP TPGRGRLCRR GCVQALALAF ALRSWRPPGT EVTSQGPRQP SSSGAKRRRL RAALGPQPTR SALRFPSASP GSLKAKQSMA GIPGRESNAP SVPTVSLLPG APGGNASSRT EAQVPNGQGS PGGCVCSSQA SPAPRAAAPP RAARGPTPRT EE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TP53I13 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product TP53I13 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    TP53I13 (Tumor Protein P53 Inducible Protein 13 (TP53I13))
    Andere Bezeichnung
    TP53I13 (TP53I13 Produkte)
    Synonyme
    2410019G02Rik Protein, Tp53i13 Protein, DSCP1 Protein, RGD1559818 Protein, Trp53i13 Protein, transformation related protein 53 inducible protein 13 Protein, tumor protein p53 inducible protein 13 Protein, Trp53i13 Protein, TP53I13 Protein, Tp53i13 Protein
    Hintergrund
    May act as a tumor suppressor. Inhibits tumor cell growth, when overexpressed. {ECO:0000269|PubMed:14767535}.
    Molekulargewicht
    31.0 kDa Including tag.
    UniProt
    Q8NBR0
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