Mastermind-Like Domain Containing 1 (MAMLD1) (AA 1-774) protein (His tag)

Details zu Produkt Nr. ABIN3083231, Anbieter: Anmelden zum Anzeigen
Proteinname
  • CG1
  • CXorf6
  • F18
  • HYSP2
  • G630014P10Rik
  • mastermind-like domain containing 1
  • MAMLD1
  • Mamld1
Proteineigenschaft
AA 1-774
1
1
1
Spezies
Human
2
1
Quelle
Insektenzellen
1
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
His tag
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Sequenz MDDWKSRLVI KSMLPHFAMV GNRQEPRKLQ ESGKKPSWME EEDLSFLYKS SPGRKHQGTV KRRQEEDHFQ FPDMADGGYP NKIKRPCLED VTLAMGPGAH PSTACAELQV PPLTINPSPA AMGVAGQSLL LENNPMNGNI MGSPFVVPQT TEVGLKGPTV PYYEKINSVP AVDQELQELL EELTKIQDPS PNELDLEKIL GTKPEEPLVL DHPQATLSTT PKPSVQMSHL ESLASSKEFA SSCSQVTGMS LQIPSSSTGI SYSIPSTSKQ IVSPSSSMAQ SKSQVQAMLP VALPPLPVPQ WHHAHQLKAL AASKQGSATK QQGPTPSWSG LPPPGLSPPY RPVPSPHPPP LPLPPPPPPF SPQSLMVSCM SSNTLSGSTL RGSPNALLSS MTSSSNAALG PAMPYAPEKL PSPALTQQPQ FGPQSSILAN LMSSTIKTPQ GHLMSALPAS NPGPSPPYRP EKLSSPGLPQ QSFTPQCSLI RSLTPTSNLL SQQQQQQQQQ QQANVIFKPI SSNSSKTLSM IMQQGMASSS PGATEPFTFG NTKPLSHFVS EPGPQKMPSM PTTSRQPSLL HYLQQPTPTQ ASSATASSTA TATLQLQQQQ QQQQQQPDHS SFLLQQMMQQ PQRFQRSVAS DSMPALPRQG CCHLFAWTSA ASSVKPQHQH GNSFTSRQDP QPGDVSPSNI THVDKACKLG EARHPQVSLG RQPPSCQALG SESFLPGSSF AHELARVTSS YSTSEAAPWG SWDPKAWRQV PAPLLPSCDA TARGTEIRSY GNDP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human MAMLD1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Reinigung Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin free.
Güteklasse Crystallography grade
Hintergrund Transactivates the HES3 promoter independently of NOTCH proteins. HES3 is a non-canonical NOTCH target gene which lacks binding sites for RBPJ. {ECO:0000269|PubMed:18162467}.
Molekulargewicht 84.2 kDa Including tag.
UniProt Q13495
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Kommentare

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Mastermind-Like Domain Containing 1 (MAMLD1) (AA 1-774) protein (His tag) (ABIN3083231) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Haben Sie etwas anderes gesucht?