ZMPSTE24 Protein (AA 1-475) (rho-1D4 tag)
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- Target Alle ZMPSTE24 (Zmpste24) Proteine anzeigen
- ZMPSTE24 (Zmpste24) (Zinc Metalloproteinase, Ste24 (Zmpste24))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-475
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Spezies
- Human
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses ZMPSTE24 Protein ist gelabelt mit rho-1D4 tag.
- Applikation
- Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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MGMWASLDAL WEMPAEKRIF GAVLLFSWTV YLWETFLAQR QRRIYKTTTH VPPELGQIMD SETFEKSRLY QLDKSTFSFW SGLYSETEGT LILLFGGIPY LWRLSGRFCG YAGFGPEYEI TQSLVFLLLA TLFSALTGLP WSLYNTFVIE EKHGFNQQTL GFFMKDAIKK FVVTQCILLP VSSLLLYIIK IGGDYFFIYA WLFTLVVSLV LVTIYADYIA PLFDKFTPLP EGKLKEEIEV MAKSIDFPLT KVYVVEGSKR SSHSNAYFYG FFKNKRIVLF DTLLEEYSVL NKDIQEDSGM EPRNEEEGNS EEIKAKVKNK KQGCKNEEVL AVLGHELGHW KLGHTVKNII ISQMNSFLCF FLFAVLIGRK ELFAAFGFYD SQPTLIGLLI IFQFIFSPYN EVLSFCLTVL SRRFEFQADA FAKKLGKAKD LYSALIKLNK DNLGFPVSDW LFSMWHYSHP PLLERLQALK TMKQH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ZMPSTE24 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin-free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product Zmpste24 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Kommentare
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- ZMPSTE24 (Zmpste24) (Zinc Metalloproteinase, Ste24 (Zmpste24))
- Andere Bezeichnung
- ZMPSTE24 (Zmpste24 Produkte)
- Synonyme
- DDBDRAFT_0189115 Protein, DDBDRAFT_0237846 Protein, DDB_0189115 Protein, DDB_0237846 Protein, F2N1.21 Protein, F2N1_21 Protein, STE24 Protein, FACE-1 Protein, FACE1 Protein, HGPS Protein, PRO1 Protein, Ste24p Protein, A530043O15Rik Protein, D030046F19 Protein, Face-1 Protein, MADB Protein, fi45a02 Protein, wu:fi45a02 Protein, zgc:55655 Protein, zinc metallopeptidase STE24 Protein, zinc metallopeptidase STE24 L homeolog Protein, CAAX prenyl protease Protein, Peptidase family M48 family protein Protein, zinc metallopeptidase, STE24 Protein, zinc metallopeptidase, STE24 homolog Protein, ZMPSTE24 Protein, zmpste24.L Protein, zmpste24 Protein, ATSTE24 Protein, Zmpste24 Protein
- Hintergrund
- Proteolytically removes the C-terminal three residues of farnesylated proteins. Acts on lamin A/C.
- Molekulargewicht
- 56.0 kDa Including tag.
- UniProt
- O75844
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