SERPINH1 Protein (AA 19-418) (His tag)

Produktnummer ABIN3076875

Produktdetails

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 19-418

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SERPINH1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: AEVKKPAAAA APGTAEKLSP KAATLAERSA GLAFSLYQAM AKDQAVENIL VSPVVVASSL GLVSLGGKAT TASQAKAVLS AEQLRDEEVH AGLGELLRSL SNSTARNVTW KLGSRLYGPS SVSFADDFVR SSKQHYNCEH SKINFRDKRS ALQSINEWAA QTTDGKLPEV TKDVERTDGA LLVNAMFFKP HWDEKFHHKM VDNRGFMVTR SYTVGVMMMH RTGLYNYYDD EKEKLQIVEM PLAHKLSSLI ILMPHHVEPL ERLEKLLTKE QLKIWMGKMQ KKAVAISLPK GVVEVTHDLQ KHLAGLGLTE AIDKNKADLS RMSGKKDLYL ASVFHATAFE LDTDGNPFDQ DIYGREELRS PKLFYADHPF IFLVRDTQSG SLLFIGRLVR PKGDKMRDEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SERPINH1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Andere Bezeichnung: SERPINH1 (SERPINH1 Produkte)

Synonyme: AsTP3 Protein, CBP1 Protein, CBP2 Protein, HSP47 Protein, OI10 Protein, PPROM Protein, RA-A47 Protein, SERPINH2 Protein, gp46 Protein, SERPINH1 Protein, DKFZp468B197 Protein, cb216 Protein, hsp Protein, hsp47 Protein, id:ibd5060 Protein, wu:fc56b02 Protein, BERF-1 Protein, Cbp1 Protein, Cbp2 Protein, Hsp47 Protein, J6 Protein, Serpinh2 Protein, colligin Protein, astp3 Protein, cbp1 Protein, cbp2 Protein, pig14 Protein, pprom Protein, ra-a47 Protein, serpinh2 Protein, serpin family H member 1 Protein, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1b Protein, serpin H1 Protein, selenoprotein K Protein, serine (or cysteine) peptidase inhibitor, clade H, member 1 Protein, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) L homeolog Protein, SERPINH1 Protein, serpinh1b Protein, LOC100009353 Protein, Selenok Protein, Serpinh1 Protein, serpinh1.L Protein

Substanzklasse: Viral Protein

Hintergrund: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.

Molekulargewicht: 45.5 kDa Including tag.

UniProt: P50454