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WDR20 Protein (AA 2-569) (His tag,GST tag)

Crystallography grade WDR20 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3075585
  • Target Alle WDR20 Proteine anzeigen
    WDR20 (WD Repeat Domain 20 (WDR20))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-569
    Spezies
    Human
    Quelle
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses WDR20 Protein ist gelabelt mit His tag,GST tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSATEG GGKEMNEIKT QFTTREGLYK LLPHSEYSRP NRVPFNSQGS NPVRVSFVNL NDQSGNGDRL CFNVGRELYF YIYKGVRKAA DLSKPIDKRI YKGTQPTCHD FNHLTATAES VSLLVGFSAG QVQLIDPIKK ETSKLFNEER LIDKSRVTCV KWVPGSESLF LVAHSSGNMY LYNVEHTCGT TAPHYQLLKQ GESFAVHTCK SKSTRNPLLK WTVGEGALNE FAFSPDGKFL ACVSQDGFLR VFNFDSVELH GTMKSYFGGL LCVCWSPDGK YIVTGGEDDL VTVWSFVDCR VIARGHGHKS WVSVVAFDPY TTSVEEGDPM EFSGSDEDFQ DLLHFGRDRA NSTQSRLSKR NSTDSRPVSV TYRFGSVGQD TQLCLWDLTE DILFPHQPLS RARTHTNVMN ATSPPAGSNG NSVTTPGNSV PPPLPRSNSL PHSAVSNAGS KSSVMDGAIA SGVSKFATLS LHDRKERHHE KDHKRNHSMG HISSKSSDKL NLVTKTKTDP AKTLGTPLCP RMEDVPLLEP LICKKIAHER LTVLIFLEDC IVTACQEGFI CTWGRPGKVV SFNPHHHHHH
    Sequence including N-terminal GST tag and C-terminal His-tag.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human WDR20 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: N-terminal GST tag, C-terminal His Tag

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    WDR20 (WD Repeat Domain 20 (WDR20))
    Andere Bezeichnung
    WDR20 (WDR20 Produkte)
    Synonyme
    DMR Protein, 2310040A13Rik Protein, AI549910 Protein, WDR20 Protein, Wdr20 Protein, wdr20 Protein, WD repeat domain 20 Protein, WD repeat domain 20a Protein, WD repeat domain 20 S homeolog Protein, WD repeat domain 20b Protein, WDR20 Protein, Wdr20 Protein, wdr20 Protein, wdr20a Protein, wdr20.S Protein, wdr20b Protein
    Molekulargewicht
    63.7 kDa Including tag.
    UniProt
    Q8TBZ3
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