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VPS28 Protein (AA 1-221) (His tag)

Crystallography grade VPS28 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, Crys, WB
Produktnummer ABIN3075431
  • Target Alle VPS28 Proteine anzeigen
    VPS28 (Vacuolar Protein Sorting 28 (VPS28))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-221
    Spezies
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 2
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses VPS28 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), ELISA, Crystallization (Crys), Western Blotting (WB)
    Sequenz
    MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human VPS28 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Kommentare

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    VPS28 (Vacuolar Protein Sorting 28 (VPS28))
    Andere Bezeichnung
    VPS28 (VPS28 Produkte)
    Synonyme
    CG12770 Protein, DmVps28 Protein, Dmel\\CG12770 Protein, Dvps28 Protein, ESCRT-I Protein, ESCRT-II Protein, dVps28 Protein, dvps28 Protein, l(2)k16503 Protein, vps28 Protein, VPS28 Protein, GB12925 Protein, VPL13 Protein, VPT28 Protein, DDBDRAFT_0186436 Protein, DDBDRAFT_0234023 Protein, DDB_0186436 Protein, DDB_0234023 Protein, 1110014J03Rik Protein, AI847241 Protein, D730005C08Rik Protein, zgc:66078 Protein, Vacuolar protein sorting 28 Protein, VPS28, ESCRT-I subunit Protein, vacuolar protein sorting 28 homolog Protein, vacuolar protein sorting-associated protein 28 homolog Protein, ESCRT-I subunit protein VPS28 Protein, ESCRT I complex subunit Vps28 Protein, transport of soluble vacuolar hydrolase precursors Protein, subunit of the ESCRT-I complex Protein, vacuolar protein sorting-associated protein Protein, vacuolar protein sorting 28 family protein Protein, vacuolar protein sorting-associated protein 28 Protein, Vacuolar protein sorting-associated protein 28 homolog Protein, vacuolar protein sorting 28 Protein, vacuolar protein sorting 28 (yeast) Protein, vacuolar protein sorting 28 homolog S homeolog Protein, Vps28 Protein, VPS28 Protein, vps28 Protein, LOC408783 Protein, CpipJ_CPIJ003920 Protein, LACBIDRAFT_399656 Protein, PTRG_04781 Protein, MCYG_00028 Protein, PITG_04534 Protein, Tsp_08691 Protein, Tsp_08687 Protein, Tsp_08688 Protein, vps-28 Protein, vps28.S Protein
    Hintergrund
    Component of the ESCRT-I complex, a regulator of vesicular trafficking process. {ECO:0000269|PubMed:11916981}.
    Molekulargewicht
    26.4 kDa Including tag.
    UniProt
    Q9UK41
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