TAF8 Protein (AA 2-310) (His tag)

Produktnummer ABIN3073808

Produktdetails

Target: TAF8 (TAF8 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 43kDa (TAF8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-310

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses TAF8 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: ADAAATAGAG GSGTRSGSKQ STNPADNYHL ARRRTLQVVV SSLLTEAGFE SAEKASVETL TEMLQSYISE IGRSAKSYCE HTARTQPTLS DIVVTLVEMG FNVDTLPAYA KRSQRMVITA PPVTNQPVTP KALTAGQNRP HPPHIPSHFP EFPDPHTYIK TPTYREPVSD YQVLREKAAS QRRDVERALT RFMAKTGETQ SLFKDDVSTF PLIAARPFTI PYLTALLPSE LEMQQMEETD SSEQDEQTDT ENLALHISME DSGAEKENTS VLQQNPSLSG SRNGEENIID NPYLRPVKKP KIRRKKSLS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TAF8 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TAF8 (TAF8 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 43kDa (TAF8))

Andere Bezeichnung: TAF8 (TAF8 Produkte)

Synonyme: CG7128 Protein, Dmel\\CG7128 Protein, HL-VIII Protein, Prodos Protein, TAF Protein, TAF8 Protein, TAF[[II]] Protein, TFIID Protein, dmTAF8 Protein, l(1)16Fg Protein, pds Protein, pro Protein, prod Protein, tbnl Protein, zgc:56401 Protein, zgc:77837 Protein, 43 Protein, II Protein, TAFII-43 Protein, TAFII43 Protein, TBN Protein, tbn Protein, AW260255 Protein, Tbn Protein, TBP-associated factor 8 Protein, TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor Protein, TATA-box binding protein associated factor 8 Protein, TATA-box binding protein associated factor 8 S homeolog Protein, Taf8 Protein, taf8 Protein, TAF8 Protein, taf8.S Protein

Hintergrund: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity). {ECO:0000250, ECO:0000269|PubMed:14580349}.

Molekulargewicht: 35.1 kDa Including tag.

UniProt: Q7Z7C8

Pathways: Maintenance of Protein Location