RUNX1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2731253

Produktdetails

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Transcript Variant 2

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses RUNX1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human RUNX1 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: RUNX1 Activity verified in a DNA-binding assay:

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Mochin, Underwood, Cooper, McLenithan, Pierce, Nalvarte, Arbiser, Karlsson, Moise, Moskovitz, Passaniti: "Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells." in: Microvascular research, Vol. 97, pp. 55-64, (2014) (PubMed).

Antigendetails

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Andere Bezeichnung: Runx1 (RUNX1 Produkte)

Synonyme: RUNX1 Protein, runx1 Protein, AI462102 Protein, AML1 Protein, Cbfa2 Protein, Pebp2a2 Protein, Pebpa2b Protein, AML1-EVI-1 Protein, AMLCR1 Protein, CBFA2 Protein, EVI-1 Protein, PEBP2aB Protein, runxa Protein, Runx-1 Protein, XAML Protein, Xaml1 Protein, aml Protein, aml-1 Protein, aml1 Protein, aml1-evi-1 Protein, amlcr1 Protein, cbfa2 Protein, evi-1 Protein, pebp2ab Protein, Aml1 Protein, uncharacterized LOC473981 Protein, runt-related transcription factor Protein, runt related transcription factor 1 Protein, runt-related transcription factor 1 Protein, runt related transcription factor 1 L homeolog Protein, LOC473981 Protein, runt Protein, Runx1 Protein, RUNX1 Protein, runx1 Protein, runx1.L Protein

Hintergrund: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 48.6 kDa

NCBI Accession: NP_001001890