Recombinant MMSET / WHSC1 - SET peptide that includes amino acids 942-1240 that contains the SET domain sequence of the human MMSET (NSD2) protein (accession number NP_579877.1) was expressed in E. coli cells and contains an N-terminal GST tag with an observed molecular weight of 60 kDa.
Crystallography grade
WHSC1
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, Crys, SDS
WHSC1
Spezies: Human
Wirt: Baculovirus
Recombinant
EAA, ScA
Applikationshinweise
Recombinant MMSET / WHSC1 - SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: Histone methyltransferase with histone H3 'Lys-36' (H3K36me) methyltransferase activity. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl MMSET / WHSC1 - SET at 2 hours at room temperature. Activity was detected by fluorography.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Lagerung
-80 °C
Informationen zur Lagerung
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
MMSET (Multiple Myeloma SET domain-containing protein) / WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1, also known as NSD2, Nuclear Receptor binding SET domain protein 2, TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The MMSET / WHSC1 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15 % of patients with Multiple Myeloma (MM), a chromosomal translocation leads to MMSET / WHSC1 gene being aberrantly placed under the control of the IgH intronic μ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in MMSET / WHSC1 expression in a myeloma cell line suppressed its growth, suggesting a role for MMSET in maintaining the transformed state. MMSET / WHSC1 - SET includes the SET domain of MMSET (NSD2). The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in the Drosophila proteins Su(var)3-9, EZH2 and TrxG and plays a crucial role in substrate recognition and enzymatic activity.