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Histone 3 Protein (H3) (H3K23me3)

H3 Spezies: Xenopus laevis Wirt: Escherichia coli (E. coli) Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S
Produktnummer ABIN2669552
  • Target Alle Histone 3 (H3) Proteine anzeigen
    Histone 3 (H3)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    H3K23me3
    Spezies
    • 19
    • 16
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Xenopus laevis
    Quelle
    • 38
    • 6
    Escherichia coli (E. coli)
    Applikation
    Positive Control (PC), Substrate (S)
    Produktmerkmale
    Recombinant Xenopus laevis Histone H3 trimethyl Lys23 (H3K23me3) is produced in E. coli and purified using FPLC. The protein contains a substitution of cysteine to alanine at amino acid 110. Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). This specific chemical treatment enables the site and degree of methylation to be controlled precisely. Each methylation reaction is over 99 % complete, as verified by high-resolution ESI-TOF mass spectrometry. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE. The molecular weight of the recombinant histone is 15,299 Daltons.
    Aufreinigung
    Purified using FPLC
    Reinheit
    The recombinant histone is >98 % pure by SDS-PAGE.
    Top Product
    Discover our top product H3 Protein
  • Applikationshinweise
    Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
    Handhabung
    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
    Lagerung
    -20 °C/-80 °C
    Informationen zur Lagerung
    Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
  • Target
    Histone 3 (H3)
    Andere Bezeichnung
    Histone H3 (H3 Produkte)
    Synonyme
    H-3 Protein, histocompatibility 3 Protein, H3 Protein
    Hintergrund
    Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
    Molekulargewicht
    The molecular weight of the recombinant histone is 15,299 Daltons.
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