Nicotinamide phosphoribosyltransferase (NAMPT) (AA 27-491), (N-Term) (Active) Protein

Details zu Produkt Nr. ABIN2667609, Anbieter: Anmelden zum Anzeigen
Proteinname
  • PBEF1
  • pbef
  • pbef1
  • 1110035O14Rik
  • PBEF
  • VF
  • VISFATIN
  • AI314458
  • AI480535
  • NAmPRTase
  • Pbef
  • Pbef1
  • Visfatin
  • visfatin
  • nicotinamide phosphoribosyltransferase
  • Nicotinamide phosphoribosyltransferase
  • NAMPT
  • nampt
  • Smon_0680
  • Mrub_2845
  • Cseg_3704
  • BC1002_3997
  • Ftrac_2686
  • Varpa_5681
  • Celal_3900
  • Deima_0880
  • BC1001_3724
  • Celly_1696
  • Clole_1542
  • Psed_2146
  • Psed_2158
  • Tsp_05934
  • Nampt
Proteineigenschaft
AA 27-491, N-Term
19
5
1
1
1
Spezies
Human
33
14
8
1
1
Quelle
Escherichia coli (E. coli)
34
4
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Reinheit >98 % , as determined by Coomassie stained SDS-PAGE.
Endotoxin-Niveau

Less than 0.1 ng per μg of protein.

Hintergrund Visfatin, also known as a pre-B cell colony-enhancing factor, is a 55 kD protein of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. This protein is produced and secreted primarily by white adipose tissue. It is also expressed by neurons within the arcuate nucleus of the hypothalamus, a brain area that plays a pivotal role in the regulation of energy metabolism. Although the metabolic function of Visfatin is still unknown, it appears that this adipocytokine might play an important role in the regulation of body weight as an afferent signal reflecting excess body fat. Importantly, Visfatin binds the insulin receptor at a site distinct from that of insulin and causes hypoglycemia by reducing glucose release from liver cells and stimulating glucose utilization in adipocytes and myocytes. Other major functions of Visfatin include enhancing phagocytosis, inducing production of CCL2 and IL-6 in umbilical cord endothelial cells, and upregulating the expression of VEGF. The expression of Visfatin is upregulated by hypoxia, inflammation and hyperglycemia and downregulated by insulin, somatostatin and statins.
Molekulargewicht The 466 amino acid recombinant protein has a predicted molecular mass of approximately 52.6 kDa. The predicted N-terminal amino acid is Met.
Forschungsgebiet Hormones, Cytokines
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Biological activity: Recombinant Visfatin is able to stimulate proliferation of RPMI 8226 cells with an ED50 of 15.0 - 20.0 ng/ml, corresponding to a specific activity of 5.0 x 104 - 6.67 x 104 units/mg.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. Note: Due to solubility reasons the protein should be kept at low pH . It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
Buffer Lyophilized
Handhabung Avoid repeated freeze/thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung Unopened vial can be stored at -20°C or -70°C.
Allgemeine Veröffentlichungen Sommer, Kralisch, Kloting, Kamprad, Schrock, Kratzsch, Tonjes, Lossner, Bluher, Stumvoll, Fasshauer: "Visfatin is a positive regulator of MCP-1 in human adipocytes in vitro and in mice in vivo." in: Obesity (Silver Spring, Md.), Vol. 18, Issue 8, pp. 1486-92, 2010 (PubMed).

Chang, Chang, Lee, Chuang: "The relationship of visfatin/pre-B-cell colony-enhancing factor/nicotinamide phosphoribosyltransferase in adipose tissue with inflammation, insulin resistance, and plasma lipids." in: Metabolism: clinical and experimental, Vol. 59, Issue 1, pp. 93-9, 2009 (PubMed).

Sommer, Garten, Petzold, Beck-Sickinger, Blüher, Stumvoll, Fasshauer: "Visfatin/PBEF/Nampt: structure, regulation and potential function of a novel adipokine." in: Clinical science (London, England : 1979), Vol. 115, Issue 1, pp. 13-23, 2008 (PubMed).

Stephens, Vidal-Puig: "An update on visfatin/pre-B cell colony-enhancing factor, an ubiquitously expressed, illusive cytokine that is regulated in obesity." in: Current opinion in lipidology, Vol. 17, Issue 2, pp. 128-31, 2006 (PubMed).