Oncostatin M (OSM) (AA 26-234) (Active) Protein

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Proteinname
  • OSM
  • OncoM
  • oncostatin M
  • oncostatin M-like
  • oncostatin-M-like
  • OSM
  • Osm
  • LOC100342491
  • LOC100152038
Proteineigenschaft
AA 26-234
7
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Spezies
Human
59
9
5
1
Quelle
Escherichia coli (E. coli)
48
6
3
3
1
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Western Blotting (WB)
Optionen
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Reinheit >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Endotoxin-Niveau

Less than 0.1 ng per μg of protein.

Hintergrund OSM is a member of gp130 family of cytokines and shares significant similarities in amino acid sequence and secondary structure with leukemia inhibitory factor (LIF). Human OSM can signal through two types of receptor complexes: type I receptor (gp130/LIFR) and type II receptor (gp130/OSMRβ). Mouse OSM can only signal through type II receptor. Binding of human OSM to gp130/LIFRa complex can activate JAK1, JAK2 and Tyk2 pathways. Binding of human OSM to another receptor, gp130/OSMRβ, can activate STAT5, STAT6, PKC delta and PI3K/Akt pathways. Proteolytic cleavage of the C-terminal hydrophilic domain can significantly increase OSM activity. OSM is a pleiotropic cytokine and its activity is dependent on different tissues. It has been shown that OSM is involved in differentiation, cell proliferation, hematopoiesis, and inflammation. OSM can regulate cell proliferation through VEGF-A or Cyclin D1. OSM can regulate inflammation via induction of other cytokines and their receptors. OSM can also regulate extracellular matrix remodeling through induction of MMP-1 and TIMP-1. OSM regulates hematopoiesis by stimulating stromal cells to provide proper microenvironment. In bone marrow, OSM can also retain hematopoietic progenitors by regulating G-CSF and SDF-1 levels. OSM has also been implicated in CNS development, fat turnover, and liver regeneration.
Molekulargewicht The 210 amino acid recombinant protein has a predicted molecular mass of approximately 24 kDa. The predicted N-terminal amino acid is Met.
Forschungsgebiet Immunology, Innate Immunity, Cytokines, Signaling, Receptors
Pathways JAK-STAT Signalweg, Negative Regulation of Hormone Secretion
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Biological activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0 x 105 units/mg.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
Buffer Lyophilized, carrier-free.
Handhabung Avoid repeated freeze/thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung Unopened vial can be stored at -20°C or -70°C.
Allgemeine Veröffentlichungen Richards: "The enigmatic cytokine oncostatin m and roles in disease." in: ISRN inflammation, Vol. 2013, pp. 512103, 2014 (PubMed).

Chen, Benveniste: "Oncostatin M: a pleiotropic cytokine in the central nervous system." in: Cytokine & growth factor reviews, Vol. 15, Issue 5, pp. 379-91, 2004 (PubMed).

Tanaka, Miyajima: "Oncostatin M, a multifunctional cytokine." in: Reviews of physiology, biochemistry and pharmacology, Vol. 149, pp. 39-52, 2003 (PubMed).

Liu, Modrell, Aruffo, Scharnowske, Shoyab: "Interactions between oncostatin M and the IL-6 signal transducer, gp130." in: Cytokine, Vol. 6, Issue 3, pp. 272-8, 1994 (PubMed).

Gearing, Comeau, Friend, Gimpel, Thut, McGourty, Brasher, King, Gillis, Mosley: "The IL-6 signal transducer, gp130: an oncostatin M receptor and affinity converter for the LIF receptor." in: Science (New York, N.Y.), Vol. 255, Issue 5050, pp. 1434-7, 1992 (PubMed).