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PF4 Protein (AA 32-101)

PF4 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 97 % , as determined by Coomassie stained SDS-PAGE. FACS Active
Produktnummer ABIN2666881
  • Target Alle PF4 Proteine anzeigen
    PF4 (Platelet Factor 4 (PF4))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 32-101
    Spezies
    • 19
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Quelle
    • 27
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Applikation
    Flow Cytometry (FACS)
    Reinheit
    > 97 % , as determined by Coomassie stained SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Biological activity: The ED50 is 2 - 5 μg/ml, corresponding to a specific activity 2 - 5 x 102 units/mg, as determined by a dose-dependent inhibition of human FGF-basic-dependent proliferation of HUVEC cells.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Buffer
    0.22 μm filtered protein solution is in PBS, pH 7.4.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Target
    PF4 (Platelet Factor 4 (PF4))
    Andere Bezeichnung
    CXCL4 (PF4 Produkte)
    Synonyme
    CXCL4 Protein, PF-4 Protein, SCYB4 Protein, Cxcl4 Protein, Scyb4 Protein, Pf4a Protein, RATPF4A Protein, PF4A Protein, CXCL4L1 Protein, CXCL4V1 Protein, PF4-ALT Protein, SCYB4V1 Protein, PF4 Protein, platelet factor 4 Protein, platelet factor 4 variant 1 Protein, PF4 Protein, Pf4 Protein, LOC703451 Protein, PF4V1 Protein, LOC100524373 Protein, LOC102181854 Protein, LOC100630489 Protein
    Hintergrund
    CXCL4, also known as platelet factor 4 (PF-4), is one of the most abundant platelet chemokines and has several biological functions depending on the cell type. CXCL4 is predominantly synthesized in megakaryocytes, stored in the α-granules of platelets, and released as a tetramer at micromolar concentrations upon platelet activation. CXCL4 has both procoagulant and anticoagulant activities. CXCL4 can bind heparin and neutralize the anticoagulant effect of heparin. On the other hand, CXCL4 inhibits factor XII, and vitamin K dependent coagulation factor, and stimulates activated protein C generation. CXCL4 is a potent tumor inhibitor because it can inhibit endothelial cell migration, proliferation, and in vivo angiogenesis through interfering with the angiogenic effect of growth factors such as FGF and VEGF. CXCL4 binds directly to FGF-β and inhibits its dimerization, which is required for receptor activation. Also, it has been suggested that binding of CXCL4 to polysulfated GAGs can interfere with activation of VEGFR and FGFR. CXCL4 also promotes inflammatory responses. For example, CXCL4 can activate granulocytes and induce monocytes to differentiate into macrophages and antigen-presenting cells. It has been reported that CXCL4 is deposited early in atherosclerotic lesions, and the presence of CXCL4 at atherosclerotic lesions has been associated with plaque progression. Genetic deletion of CXCL4 in Apo-E-/- mice is accompanied with reduced atherogenesis. CXCL4L1, a CXCL4 variant which differs in only three amino acids in its mature form, has been identified. CXCL4 and CXCL4L1 have different subcellular localization and are secreted in a differentially regulated manner. Human CXCL4 binds to CXCR3A and CXCR3B receptor variants, and it should be noted that variant B has not been described in mice. CXCR3A acts as a chemotactic receptor for human cells via CXCL4, and CXCR3B has an antiapoptotic effect in endothelial cells. CXCR3A might be responsible for the chemotactic effect of CXCL4 in mice.
    Molekulargewicht
    The 70 amino acid recombinant protein has a predicted molecular mass of approximately 7.8 kDa. The DTT-reduced and non-reduced protein migrates at approximately 11 kDa by SDS-PAGE. The predicted N-terminal amino acid is Glu.
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