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Osteoprotegerin Protein (AA 22-401, C-Term)

TNFRSF11B Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % , as determined by Coomassie stained SDS-PAGE. CyTOF, FACS Active
Produktnummer ABIN2666865
  • Target Alle Osteoprotegerin (TNFRSF11B) Proteine anzeigen
    Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 22-401, C-Term
    Spezies
    • 17
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    Maus
    Quelle
    • 12
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Applikation
    Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS)
    Reinheit
    > 95 % , as determined by Coomassie stained SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

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    Discover our top product TNFRSF11B Protein
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Biological activity: ED50 = 30 - 90 ng/ml, corresponding to a specific activity of 1 - 3 x 104 units/mg, as determined by inhibition of cytotoxic effect of human TRAIL on L929 cells.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Buffer
    0.22 μm filtered protein solution is in PBS.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Target
    Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))
    Andere Bezeichnung
    TNFRSF11B (TNFRSF11B Produkte)
    Synonyme
    CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, OCIF Protein, OPG Protein, TR1 Protein, Opg Protein, ocif Protein, opg Protein, tr1 Protein, TNF superfamily member 11 Protein, TNF receptor superfamily member 11b Protein, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) Protein, TNF receptor superfamily member 11B Protein, tumor necrosis factor receptor superfamily member 11b S homeolog Protein, tumor necrosis factor receptor superfamily, member 11b Protein, TNFSF11 Protein, TNFRSF11B Protein, Tnfrsf11b Protein, tnfrsf11b.S Protein
    Substanzklasse
    Chemical
    Hintergrund
    TNFRSF11B is a member of the TNFR family, which lacks the transmembrane domain and acts as a secreted decoy receptor with no direct signaling capacity. It plays a key role in the physiological regulation of osteoclastic bone resorption, postnatal bone formation, and pathological calcification of arteries. TNFRSF11B binds to its natural ligand, RANKL (OPGL), which prevents the interaction of RANKL with its receptor, present on osteoclasts, therefore inhibiting osteoclast differentiation, activation, and survival. Overexpression of TNFRSF11B in transgenic mice leads to deep osteoporosis secondary to an almost total lack of osteoclasts. In addition, TNFRSF11B-deficient mice develop early onset osteoporosis and calcification of the aorta and renal arteries, sites which have endogenous TNFRSF11B expression in normal animals. EBF family member, EBF2 activates TNFRSF11B gene transcription in a synergistic manner with Wnt/β-catenin signaling transcription factor LEF1. In addition to RANKL, TNFRSF11B binds and neutralizes the pro-apoptotic actions of TNF-related apoptosis-inducing ligand (TRAIL) expressed by vascular smooth muscle cells (VSMCs) and T cells. Lastly, TNFRSF11B is involved in B cell development and function. High levels of TNFRSF11B have been detected in serum of patients with cardiovascular diseases, diabetes, and chronic renal failure.
    Molekulargewicht
    The 620 amino acid recombinant protein has a predicted molecular mass of approximately 70.6 kDa. The DTT-reduced and non-reduced protein migrate at approximately 100 kDa and 200 kDa, respectively, by SDS-PAGE. The predicted N-terminal amino acid is Glu.
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