BAFF Protein (AA 127-309)

Produktnummer ABIN2666770

Produktdetails

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 127-309

Spezies: Maus

Quelle: CHO Cells

Applikation: ELISA, Flow Cytometry (FACS)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 1 EU per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 0.5 - 3 ng/ml, corresponding to a specific activity of 0.33 - 2.0 x 106 units/mg, as determined by the dose dependent stimulation of B cells proliferation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS pH 6.5.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Andere Bezeichnung: BAFF (TNFSF13B Produkte)

Synonyme: BAFF Protein, BLYS Protein, CD257 Protein, DTL Protein, TALL-1 Protein, TALL1 Protein, THANK Protein, TNFSF20 Protein, ZTNF4 Protein, BAFF-R Protein, BAFFR Protein, BROMIX Protein, CD268 Protein, CVID4 Protein, prolixin Protein, TNFSF13B Protein, RGD1561519 Protein, RGD1560810 Protein, BLyS Protein, D8Ertd387e Protein, zTNF4 Protein, 2010006P15Rik Protein, Baffr Protein, Bcmd Protein, Bcmd-1 Protein, Bcmd1 Protein, Lvis22 Protein, zgc:172115 Protein, TNF superfamily member 13b Protein, TNF receptor superfamily member 13C Protein, tumor necrosis factor (ligand) superfamily, member 13b Protein, tumor necrosis factor receptor superfamily, member 13c Protein, tumor necrosis factor superfamily member 13b Protein, TNFSF13B Protein, TNFRSF13C Protein, Tnfsf13b Protein, Tnfrsf13c Protein, tnfsf13b Protein

Hintergrund: BAFF is a TNF cytokine member (a type II membrane protein) that acts in both a membrane-bound form and soluble cytokine form. The extracellular domain of BAFF shows high homology with APRIL. The soluble form is derived by a cleavage in a furin consensus site. In human neutrophils treated with G-CSF, BAFF is processed intracellularly by a furin-type convertase. BAFF acts mainly as a soluble trimer and binds to three receptors, with decreasing affinity in the following order: BAFFR > TACI > BCMA. Mice deficient in BAFF or its receptor BAFFR exhibit an obstruction in B cell development after the transitional T1 stage and lack a marginal zone (MZ) and conventional B2 B cells. BAFFR-deficient mice and humans (patients with common variable immunodeficiency, CVID) develop severe B cell lymphopenia due to the arrest of B cell development at the transitional B cell stage. Extreme BAFF production triggers severe autoimmune disorders in mice similar to systemic lupus erythematosus and Sjögren's syndrome. BAFF has been associated with different human diseases such as pancreatic ductal adenocarcinoma (PDAC), autoimmune pancreatitis, systemic lupus erythematosus, rheumatoid arthritis, Sjögren's syndrome, lymphoid cancers, HIV infection, and B cell malignancies such as non-Hodgkin's lymphoma (NHL).

Molekulargewicht: The 204 amino acid recombinant protein has a predicted molecular mass of approximately 22.8 kDa. The DTT-reduced and non-reduced protein migrate at approximately 20-25 and 50-55 kDa by SDS-PAGE respectively. The N-terminal contains a 9His-2(SGGG)-IEGR-tag

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response