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Aggrecan Protein (ACAN) (AA 20-675)

ACAN Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % , as determined by Coomassie stained SDS-PAGE. ELISA, FACS Active
Produktnummer ABIN2666766
  • Target Alle Aggrecan (ACAN) Proteine anzeigen
    Aggrecan (ACAN)
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 20-675
    Spezies
    • 6
    • 3
    • 3
    • 3
    • 1
    Human
    Quelle
    • 12
    • 2
    • 1
    • 1
    HEK-293 Cells
    Applikation
    ELISA, Flow Cytometry (FACS)
    Reinheit
    > 95 % , as determined by Coomassie stained SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau

    Less than 0.1 EU per μg of protein as determine by the LAL method.

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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Biological activity: Recombinant human aggrecan (G1-IGD-G2) binds immobilized hyaluronan-biotin (0.1 μg/ml) on a streptavidin plate in a dose dependent manner with a ED50 of 2.5 - 9.0 μg/ml.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Konzentration
    200 μg/mL
    Buffer
    0.22 μm filtered protein solution is in PBS.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Target
    Aggrecan (ACAN)
    Andere Bezeichnung
    Aggrecan (ACAN Produkte)
    Synonyme
    AGC1 Protein, AGCAN Protein, CSPG1 Protein, CSPGCP Protein, MSK16 Protein, SEDK Protein, Agc Protein, Agc1 Protein, Cspg1 Protein, b2b183Clo Protein, cmd Protein, aggrecan Protein, aggrecan core protein Protein, ACAN Protein, Acan Protein, LOC100380783 Protein, AGC Protein
    Hintergrund
    Aggrecan, also known as aggrecan 1, is the major proteoglycan in the articular cartilage. This high molecular weight protein exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. Aggrecan plays an important role in the proper functioning of articular cartilage by providing a hydrated gel structure through its interaction with hyaluronan and link proteins, that endows the cartilage with load-bearing properties. Aggrecan is expressed by chondrocytes and found to be crucial in chondroskeletal morphogenesis during development. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Structurally, aggrecan is composed of two globular structural domains (G1 and G2) at the N-terminal end and one globular domain (G3) at the C-terminal end, separated by a large extended domain (CS) heavily modified with glycosaminoglycans. G1 and G2 domains of aggrecan are separated by the interglobular domain (IGD). Biologically, the G1 domain interacts with hyaluronan acid and link proteins, forming stable ternary complexes in the extracellular matrix (ECM). The G2 domain is homologous to the tandem repeats of G1 and link proteins, and is involved in product processing. G3 makes up the carboxyl terminus of the core protein, and enhances glycosaminoglycan modification and product secretion. Also, the G3 domain links the proteoglycan aggregates to the ECM proteins (fibulins and tenascins). Degradation of aggrecan appears to initiate at the C-terminus. The population of aggrecan molecules without the G3 domain increases with aging.
    Molekulargewicht
    This 672 amino acid recombinant protein with a C-terminal His tag has a predicted molecular mass of approximately 74.3 kDa. The protein migrates at about 120 kDa by SDS-PAGE in DTT-reducing and non-reducing conditions. The predicted N-terminal amino acid
    Pathways
    Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
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