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TNF alpha Protein (AA 77-233)

TNF alpha Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Purity is > 98 % , as determined by Coomassie stained SDS-PAGE. FACS Active
Produktnummer ABIN2666707
  • Target Alle TNF alpha Proteine anzeigen
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 77-233
    Spezies
    • 43
    • 24
    • 12
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 61
    • 28
    • 23
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Applikation
    Flow Cytometry (FACS)
    Reinheit
    Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau

    Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Biological activity: The ED50 is 0.020-0.10 ng/ml, corresponding to a specific activity of 1-5 x 107 units/mg, as determined by a dose dependent stimulation of L929 cells treated with actinomycin D.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
    Buffer
    0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
  • Target
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Andere Bezeichnung
    TNF-alpha (TNF alpha Produkte)
    Synonyme
    DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
    Hintergrund
    TNF-α is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-α is released from cells by proteolysis of the integral membrane precursor form of TNF-α. TNF-α binding to some TNF-α receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-α is involved in the inflammatory response.
    Molekulargewicht
    The 157 amino acid recombinant protein has a predicted molecular mass of 17,352 Da. The DTT-reduced protein and non-reduced protein migrate at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Val.
    Pathways
    NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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