G-CSF Protein (AA 31-208)
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- Target Alle G-CSF (CSF3) Proteine anzeigen
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 31-208
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Spezies
- Maus
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Flow Cytometry (FACS)
- Reinheit
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
- Top Product
- Discover our top product CSF3 Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Biological activity: ED50 =0.5 - 3 ng/ml, corresponding to a specific activity of 0.33 - 2.0 x 106 units/mg, as determined by M-NFS-60 cell proliferation induced by mouse G-CSF in a dose dependent manner.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Rekonstitution
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- Buffer
- 0.22 μm filtered protein solution is in PBS.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- Target
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- Andere Bezeichnung
- G-CSF (CSF3 Produkte)
- Synonyme
- CSF3 Protein, G-CSF Protein, gcsf Protein, Csfg Protein, MGI-IG Protein, C17orf33 Protein, CSF3OS Protein, GCSF Protein, Gcsf Protein, colony stimulating factor 3 Protein, colony stimulating factor 3 (granulocyte) a Protein, colony stimulating factor 3 (granulocyte) Protein, CSF3 Protein, csf3a Protein, Csf3 Protein
- Hintergrund
- G-CSF is the key hematopoietic cytokine involved in the control of neutrophil production, therefore it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC-trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/ macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells, in addition, it is expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.
- Molekulargewicht
- The 179 amino acid recombinant protein has a predicted molecular mass of approximately 19 kDa. The DTT-reduced and non-reduced protein migrate at approximately at 20 kDa by SDS-PAGE. The N-terminal amino acid is Met.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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