Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-96) (Active) Protein

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Proteinname
  • I-309
  • P500
  • SCYA1
  • SISe
  • TCA3
  • BF534335
  • Scya1
  • Tca-3
  • CCLi6
  • chemokine
  • chemokine (C-C motif) ligand 1
  • CCL1
  • Ccl1
Proteineigenschaft
AA 24-96
9
3
1
1
1
1
Spezies
Human
29
4
Quelle
Escherichia coli (E. coli)
18
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Flow Cytometry (FACS)
Optionen
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Reinheit > 98 % , as determined by Coomassie stained SDS-PAGE.
Sterilität 0.22 μm filtered
Hintergrund CCL1 was initially identified as a secreted protein derived from activated T cells. The CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation, as a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatic patients compared with normal controls. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro, and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Also, antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.
Molekulargewicht The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.6 kDa. The DTT-reduced protein migrates at approximately 13 kDa, and the non-reduced protein migrates at approximately 15 kDa by SDS-PAGE. The N-terminal amino acid is
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Biological activity: Bioactivity was measured by its property to chemoattract mouse BW.5147.G.1.4OUAR.1 cells in a dose dependent manner.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Rekonstitution For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
Buffer 0.22 μm filtered protein solution is in PBS.
Handhabung Avoid repeated freeze/thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
Allgemeine Veröffentlichungen Mutalithas, Guillen, Raport, Kolbeck, Soler, Brightling, Pavord, Wardlaw: "Expression of CCR8 is increased in asthma." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 40, Issue 8, pp. 1175-85, 2010 (PubMed).

Gonzalo, Qiu, Lora, Al-Garawi, Villeval, Boyce, Martinez-A, Marquez, Goya, Hamid, Fraser, Picarella, Cote-Sierra, Hodge, Gutierrez-Ramos, Kolbeck, Coyle: "Coordinated involvement of mast cells and T cells in allergic mucosal inflammation: critical role of the CC chemokine ligand 1:CCR8 axis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1740-50, 2007 (PubMed).

Montes-Vizuet, Vega-Miranda, Valencia-Maqueda, Negrete-García, Velásquez, Teran: "CC chemokine ligand 1 is released into the airways of atopic asthmatics." in: The European respiratory journal, Vol. 28, Issue 1, pp. 59-67, 2006 (PubMed).

Soler, Chapman, Poisson, Wang, Cote-Sierra, Ryan, McDonald, Badola, Fedyk, Coyle, Hodge, Kolbeck: "CCR8 expression identifies CD4 memory T cells enriched for FOXP3+ regulatory and Th2 effector lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 10, pp. 6940-51, 2006 (PubMed).

Gombert, Dieu-Nosjean, Winterberg, Bünemann, Kubitza, Da Cunha, Haahtela, Lehtimäki, Müller, Rieker, Meller, Pivarcsi, Koreck, Fridman, Zentgraf, Pavenstädt, Amara, Caux, Kemeny, Alenius, Lauerma et al.: "CCL1-CCR8 interactions: an axis mediating the recruitment of T cells and Langerhans-type dendritic cells to sites of atopic skin inflammation. ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 8, pp. 5082-91, 2005 (PubMed).

Haque, Fallon, Pan, Taubman, Harpel: "Chemokine receptor-8 (CCR8) mediates human vascular smooth muscle cell chemotaxis and metalloproteinase-2 secretion." in: Blood, Vol. 103, Issue 4, pp. 1296-304, 2004 (PubMed).

Miller, Hata, De Waal Malefyt, Krangel: "A novel polypeptide secreted by activated human T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 9, pp. 2907-16, 1989 (PubMed).

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