COL18A1 Protein (AA 1154-1335, N-Term)

Produktnummer ABIN2666547

Produktdetails

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1154-1335, N-Term

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 0.5 - 3 μg/ml, as determined by inhibition of HUVEC cell proliferation induced by human FGF basic.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Andere Bezeichnung: Endostatin (COL18A1 Produkte)

Synonyme: KNO Protein, KNO1 Protein, KS Protein, cb371 Protein, cb529 Protein, fc96d06 Protein, sb:cb529 Protein, wu:fb47h10 Protein, wu:fb77g12 Protein, wu:fc50f04 Protein, wu:fc96d06 Protein, wu:fk02h12 Protein, kno Protein, kno1 Protein, collagen type XVIII alpha 1 chain Protein, collagen type XVIII alpha 1 chain a Protein, collagen, type XVIII, alpha 1 Protein, collagen type XVIII alpha 1 Protein, COL18A1 Protein, col18a1a Protein, Col18a1 Protein, col18a1 Protein

Substanzklasse: Chemical

Hintergrund: Endostatin is a C-terminal proteolytic fragment of collagen XVIII α1 (Col 18) that is localized in the vascular basement membrane of almost all epithelia and endothelia. Endostatin is produced from catalytic cleavage from MMPs, cathepsin L, and S. It is detected in circulation at a physiologic level of 20 to 50 ng/mL in serum. Crystal X-ray analysis of endostatin has shown a compact globular structure, with one face rich in arginine residues that acts as a heparin-binding motif. Endostatin interacts with multiple molecules including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, thrombospondin-1, SPARC, collagens (I, IV, and VI), the amyloid peptide Aβ (1-42), transglutaminase-2, and among others. Endostatins derived from collagens XV and XVIII differ in structure and binding properties, tissue distribution, and anti-angiogenic activity. Endostatin is an endogenous angiostatic factor that induces apoptosis in endothelial cells and inhibits endothelial cell migration in vitro. Col 18-null mice showed faster tumor growth when implanted with tumor cells that do not produce Col 18. Overexpression of circulating endostatin in transgenic mice resulted in reduced tumor growth. Experimental model of glomerulonephritis in Col 18-null mice showed that the absence of this molecule exacerbates immune-mediated glomerulonephritis.

Molekulargewicht: The 192 amino acid recombinant protein has a predicted molecular mass of approximately 21.3 kDa. The DTT-reduced and non-reduced protein migrate at approximately 23 and 20 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.