FAS Protein (AA 17-173, C-Term)

Produktnummer ABIN2666540

Produktdetails

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 17-173, C-Term

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.1 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 106 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaHPO4, 0.3 M NaCl, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: FAS (FAS Produkte)

Synonyme: ALPS1A Protein, APO-1 Protein, APT1 Protein, CD95 Protein, FAS1 Protein, FASTM Protein, TNFRSF6 Protein, AI196731 Protein, APO1 Protein, TNFR6 Protein, Tnfrsf6 Protein, lpr Protein, Fas cell surface death receptor Protein, Fas (TNF receptor superfamily member 6) Protein, FAS Protein, Fas Protein, fas Protein

Hintergrund: FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. FAS is a type I transmembrane protein and belongs to the TNF receptor superfamily. The extracellular region of FAS possesses three cystein-rich domains characteristic of the TNF superfamily, and the intracellular region which includes a death domain (DD). The binding of FASL to FAS induces conformational changes in FAS, leading to the recruitment of FADD and procaspase-8 and the assembly of the death-inducing signaling complex (DISC). Subsequently, caspase 8 is released from DISC and induces activation of caspase 3 and 7, proteolysis of cellular components, and apoptosis. Other biological effects not leading to apoptotic cell death have also been described. The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. Fas-activated DCs increase the expression of MHC II, B7 co-stimulatory molecules, and DC-lysosome-associated membrane proteins (DC-LAMP), and secrete proinflammatory cytokines such as IL-1β and TNF-α. In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34+ cells and increases their colony formation. Human activated PBMC and tumor cell lines express full-length mRNA and several mRNA FAS variants derived by alternative splicing, deletion of an exon encoding the transmembrane domain results in a soluble FAS molecule that blocks apoptosis. Patients with systemic lupus erythematosus show high levels of sFAS.

Molekulargewicht: The 397 amino acid recombinant protein has a predicted molecular mass of approximately 44.7 kDa. The DTT-reduced and non-reduced protein migrate at approximately 55 - 60 kDa and 120 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is Arg.

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity