VEGF 165 (Active) protein (Biotin)

Details zu Produkt Nr. ABIN2567017, Anbieter: Anmelden zum Anzeigen
Proteinname
Spezies
Human
33
5
4
Quelle
HEK-293 Cells
30
6
3
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Biotin
Applikation
ELISA, Western Blotting (WB)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Produktmerkmale

Measured by its binding ability in a functional ELISA. Immobilized VEGFR2/R3-Fc at 5 μg/mL (100 μL/well) can bind Biotinylated rh VEGF165 with a linear range of 0. 5-5 ng/mL.

Reinheit >95 % as determined by reduced SDS-PAGE.
Hintergrund VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers. The primary amine of rhVEGF165 was labeled by biotin without decreasing its bioactivity, and has been used successfully for easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated probes. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination.
Molekulargewicht 19 kDa (monomer)
Gen-ID 7422
NCBI Accession NP_001020539
UniProt P15692
Applikationshinweise This recombinant protein can be used for WB, ELISA. For research use only.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Haben Sie etwas anderes gesucht?