EPH Receptor B4 (EPHB4) (Active) protein (Biotin,His tag)

Details zu Produkt Nr. ABIN2444108, Anbieter: Anmelden zum Anzeigen
Proteinname
  • htk
  • myk1
  • ephB4a
  • tyro11
  • ephb4b
  • MGC130997
  • AI042935
  • Htk
  • MDK2
  • Myk1
  • Tyro11
  • HTK
  • MYK1
  • TYRO11
  • EPH receptor B4
  • EPH receptor B4 L homeolog
  • EPH receptor B4 S homeolog
  • Eph receptor B4
  • EPHB4
  • ephb4.L
  • ephb4.S
  • ephb4
  • Ephb4
Spezies
Human
24
5
Quelle
HEK-293 Cells
10
8
5
3
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses EPH Receptor B4 Protein ist gelabelt mit Biotin,His tag.
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Hersteller
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Hersteller Produkt- Nr.
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Marke MABSol®,UltraLys
Spezifität The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Produktmerkmale This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.8 kDa. The protein migrates as 65-70 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Reinheit >97 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Güteklasse Ultra Sensitive
Hintergrund Ephrin type-B receptor 4(EPHB4) is also known as HTK, MYK1 and TYRO11,is a member of Eph family. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EPHB4 binds to Ephrin-B2 and plays an essential role in vascular development. EPHB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EPHB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis and tumor growth. Incontrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EPHB4 is associated with prostate cancer and highly malignant breast cancers, accordingly, EPHB4 has potential application as a therapeutic candidate.
Molekulargewicht 57.8 kDa
NCBI Accession NP_004435
UniProt P54760
Pathways RTK Signalweg
Kommentare

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Bilder des Herstellers
SDS-PAGE (SDS) image for EPH Receptor B4 (EPHB4) (Active) protein (Biotin,His tag) (ABIN2444108) Biotinylated Human EphB4 on SDS-PAGE under reducing (R) condition. The gel was staine...