Tumor Necrosis Factor (TNF) (AA 77-233) (Active) protein (Biotin)

Details zu Produkt Nr. ABIN2181834, Anbieter: Anmelden zum Anzeigen
Proteinname
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Proteineigenschaft
AA 77-233
30
20
12
11
7
5
5
5
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
123
51
30
15
11
8
7
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
HEK-293 Cells
167
29
16
4
4
3
3
2
2
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses Tumor Necrosis Factor Protein ist gelabelt mit Biotin.
Optionen
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Bilder

Marke MABSol®,UltraLys
Spezifität The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Produktmerkmale The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.
Reinheit >95 % as determined by reduced SDS-PAGE.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Güteklasse Ultra Sensitive
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human TNFR1/CD120a Protein,C-Fc Tag (Catalog # TN1-H5251) at 5 μg/mL (100 μL/well) can bind Human TNF-alpha Protein, Biotinylated (Catalog # TNA-H8211) with a linear range of 0.8-10 ng/mL.

SDS-PAGE: The primary amine of Biotinylated TNFa was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Hintergrund Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
Molekulargewicht 17.4 kDa
NCBI Accession NP_000585
UniProt P01375
Forschungsgebiet Inflammation, Cancer, Virology
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Kommentare

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Bilder des Herstellers
SDS-PAGE (SDS) image for Tumor Necrosis Factor (TNF) (AA 77-233) (Active) protein (Biotin) (ABIN2181834) Biotinylated Human TNF-alpha on SDS-PAGE under reducing (R) condition. The gel was st...
Binding Studies (Bind) image for Tumor Necrosis Factor (TNF) (AA 77-233) (Active) protein (Biotin) (ABIN2181834) Immobilized Humira at 5 µg/mL (100 µl/well) can bind Biotinylated Human TNF-alpha wi...
Allgemeine Veröffentlichungen Dowlati, Herrmann, Swardfager, Liu, Sham, Reim, Lanctôt: "A meta-analysis of cytokines in major depression." in: Biological psychiatry, Vol. 67, Issue 5, pp. 446-57, 2010 (PubMed).